Abcam’s HRP Stability Conjugate Reagent (ab171537) was developed specifically for Horseradish Peroxidase (HRP) conjugates in solution. ab171537 is readily incorporated into virtually any assay production protocol. Conjugates can be added directly to ab171537 and are then ready for storage and use. It is also an excellent diluent, and often decreases background and increases signal for improved signal-to-noise ratios. ab171537 prevents the loss of catalytic activity while it maintains the conformation of the antibody or protein-antigen portion of the conjugate.

Recommendations for Use of ab171537 HRP Stability Conjugate Reagent

• For optimal stability and performance, use ab171537 at 100% concentration
• Dilute your HRP conjugate to optimum working concentration in the HRP Stability Conjugate Reagent solution
• Use diluted conjugate working solution in your assay as normal
• Or, preferably, store away from direct light at room temperature and use as a stock solution for optimum assay-to-assay consistency

NOTE – When storing any conjugate/ HRP Stability Conjugate Reagent solution for an extended period of time: If a phosphate buffer is used for dilution we recommend a final concentration of no more than 5 mM buffer to avoid the possibility of initiating precipitation.