Anti-iASPP antibody (ab34898)
Key features and details
- Rabbit polyclonal to iASPP
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-iASPP antibody
See all iASPP primary antibodies -
Description
Rabbit polyclonal to iASPP -
Host species
Rabbit -
Specificity
Expect reactivity against both isoform 1 and isoform 2. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow
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Immunogen
Synthetic peptide:
R EGESVTVLRR DGPEETD
, corresponding to amino acids 780-797 of Human iASPP (Isoform 1).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-iASPP antibody (ab34898)Anti-iASPP antibody (ab34898) at 1/1500 dilution (at 4°C, o/n) + 35ug of MCF7 whole cell lysate
Secondary
IRDye™800 conjugated anti-Rabbit IgG diluted 1:10000, for 45 min at RT
Predicted band size: 90 kDa
Observed band size: 102 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-iASPP antibody (ab34898)IHC image of ab34898 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab34898, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
