Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Thursday March 04, 2021

Anti-PIST antibody [EPR4080(2)] (ab133472)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4080(2)] to PIST
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-PIST antibody [EPR4080(2)]
See all PIST primary antibodies
Description

Rabbit monoclonal [EPR4080(2)] to PIST
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

A synthetic peptide corresponding to residues near the C terminus of Human PIST (UniProt Q9HD26).
Positive control
- Human fetal kidney and PC3 cell lysates; Human kidney tissue
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4080(2)
Isotype

IgG
Research areas

Western blot - Anti-PIST antibody [EPR4080(2)] (ab133472)

All lanes : Anti-PIST antibody [EPR4080(2)] (ab133472) at 1/1000 dilution

Lane 1 : Human fetal kidney lysate
Lane 2 : PC3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 51 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4080(2)] (ab133472)

Immunohistochemical analysis of PIST in paraffin embedded Human kidney tissue, using ab133472 at a dilution of 1/50-1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4080(2)] (ab133472)

ab133472 showing positive staining in Normal liver tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4080(2)] (ab133472)

ab133472 showing positive staining in Normal breast tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4080(2)] (ab133472)

ab133472 showing positive staining in Normal stomach tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-PIST antibody [EPR4080(2)] (ab133472)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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