Anti-PLK4 antibody (ab2642)
Key features and details
- Goat polyclonal to PLK4
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PLK4 antibody
See all PLK4 primary antibodies -
Description
Goat polyclonal to PLK4 -
Host species
Goat -
Tested applications
Suitable for: ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Dog
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Immunogen
Synthetic peptide:
ATCIGEKIEDFKVGN
, corresponding to N terminal amino acids 2-16 of Human PLK4. -
Positive control
- Human colon lysate
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General notes
GenBank Accession Number - NP_055079
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-PLK4 antibody (ab2642)Immunofluorescence analysis of paraformaldehyde fixed HeLa cells staining PLK4. Cells were permeabilized with 0.15% Triton. Samples were incubated with primary anitbody for 1 hour at 10µg/ml. An Alexa Fluor 488 was used as the secondary antibody. DAPI was used as a nuclear counterstain. Unimmunized goat IgG (10µg/ml) was used as the negative control.
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Western blot - Anti-PLK4 antibody (ab2642)Anti-PLK4 antibody (ab2642) at 2 µg/ml + human colon lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 109 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 1 hour
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Flow Cytometry - Anti-PLK4 antibody (ab2642)Flow Cytometry analysis of HeLa cells labeling PLK4 with ab2642 at 10ug/mL followed by Alexa Fluor 488 secondary antibody (1ug/mL) (blue line). Cells were permeabilized with 0.5% Triton. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-PLK4 antibody (ab2642)Immunofluorescence analysis of paraformaldehyde fixed A431 cells staining PLK4. Cells were permeabilized with 0.15% Triton. Samples were incubated with primary anitbody for 1 hour at 10µg/ml. An Alexa Fluor 488 was used as the secondary antibody. DAPI was used as a nuclear counterstain. Unimmunized goat IgG (10µg/ml) was used as the negative control.
