Anti-PKA R2/PKR2 (phospho S99) antibody [E151] - BSA and Azide free (ab238951)
![Anti-PKA R2/PKR2 (phospho S99) antibody [E151] - BSA and Azide free (ab238951)](https://www.abcam.com/ps/products/238/ab238951/Images/ab238951-4-ab32390IHC2.jpg "Anti-PKA R2/PKR2 (phospho S99) antibody [E151] - BSA and Azide free (ab238951)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E151] to PKA R2/PKR2 (phospho S99) - BSA and Azide free
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PKA R2/PKR2 (phospho S99) antibody [E151] - BSA and Azide free
See all PKA R2/PKR2 primary antibodies -
Description
Rabbit monoclonal [E151] to PKA R2/PKR2 (phospho S99) - BSA and Azide free -
Host species
Rabbit -
Specificity
The antibody only detects PKA R2/PKR2 phosphorylated on Serine 99. Phosphoserine 99 in Human corresponds to phosphoserine 96 in mouse.
The mouse and pig recommendation is based on the WB results. We do not guarantee IHC-P for mouse and pig.
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Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HeLa cells. IHC-P: Human lymph node metastatic tissue. Human kidney without Lambda Protein Phosphatase treatment (image A) and Rat kidney without Lambda Protein Phosphatase treatment
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General notes
ab238951 is the carrier-free version of ab32390 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab238951 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as PKA R2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E151 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 (phospho S99) antibody [E151] - BSA and Azide free (ab238951)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney without Lambda Protein Phosphatase treatment (image A) and treated with Lambda Protein Phosphatase (image B). tissue sections labeling PKA R2/PKR2 with purified ab32390 at 1:500 dilution (0.75 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 (phospho S99) antibody [E151] - BSA and Azide free (ab238951)](https://www.abcam.com/ps/products/238/ab238951/Images/ab238951-3-ab32390IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 (phospho S99) antibody [E151] - BSA and Azide free (ab238951)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney without Lambda Protein Phosphatase treatment (image A) and treated with Lambda Protein Phosphatase (image B). tissue sections labeling PKA R2/PKR2 with purified ab32390 at 1:500 dilution (0.75 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 (phospho S99) antibody [E151] - BSA and Azide free (ab238951)](https://www.abcam.com/ps/products/238/ab238951/Images/ab238951-2-ab323903.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 (phospho S99) antibody [E151] - BSA and Azide free (ab238951)ab32390, at a 1/250 dilution, staining (A) untreated (B) Phosphatase treated lymph node metastatic by immunohistochemistry, Paraffin embedded human tissue
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32390).
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![Immunocytochemistry/ Immunofluorescence - Anti-PKA R2/PKR2 (phospho S99) antibody [E151] - BSA and Azide free (ab238951)](https://www.abcam.com/ps/products/238/ab238951/Images/ab238951-317749-51.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PKA R2/PKR2 (phospho S99) antibody [E151] - BSA and Azide free (ab238951)Cell line: HeLa (human cervix adenocarcinoma)
Target AbID: Ab32390 anti-PKA R2/PKR2 (phospho S99)
Target Secondary: ab150077 AlexaFluor®488 goat anti-rabbit
Counterstain AbID: ab7291 anti-Tubulin (mouse mAb)
Target Secondary: ab150120 AlexaFluor®594 goat anti-mouse
Fixative: 4% PFA
Permeabilization: 0.1% Triton-X
Nuclear counter stain: DAPI
Other comments: Confocal image showed increased cytoplasmic staining after CA (100ng/ml, 10min) treatment on Hela cells. The LP treatment decreased the increased cytoplasmic staining caused by CA. Ab32514 was used as a Pan control for ab32390. The results showed cytoplasmic staining on untreated, CA and CA+LP treated Hela cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32390).
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![Anti-PKA R2/PKR2 (phospho S99) antibody [E151] - BSA and Azide free (ab238951)](https://www.abcam.com/ps/products/238/ab238951/Images/ab238951-5-benefits-of-recombinant-antibodies.png)
Anti-PKA R2/PKR2 (phospho S99) antibody [E151] - BSA and Azide free (ab238951)
