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Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKA

Anti-AKAP1 antibody - N-terminal (ab228875)

Anti-AKAP1 antibody - N-terminal (ab228875)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to AKAP1 - N-terminal
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-AKAP1 antibody - N-terminal
    See all AKAP1 primary antibodies
  • Description

    Rabbit polyclonal to AKAP1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Recombinant fragment within Human AKAP1 (N terminal). The exact sequence is proprietary.
    Database link: Q92667

  • Positive control

    • WB: HeLa whole cell lysate. IHC-P: Human colon carcinoma tissue. ICC/IF: HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKA

Images

  • Western blot - Anti-AKAP1 antibody - N-terminal (ab228875)
    Western blot - Anti-AKAP1 antibody - N-terminal (ab228875)
    Anti-AKAP1 antibody - N-terminal (ab228875) at 1/10000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

    Predicted band size: 97 kDa



    7.5% SDS-PAGE gel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP1 antibody - N-terminal (ab228875)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKAP1 antibody - N-terminal (ab228875)

    Paraffin-embedded human colon carcinoma tissue stained for AKAP1 with ab228875 at 1/250 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-AKAP1 antibody - N-terminal (ab228875)
    Immunocytochemistry/ Immunofluorescence - Anti-AKAP1 antibody - N-terminal (ab228875)

    Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for AKAP1 (green) using ab228875 at 1/200 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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