Anti-Dopamine Receptor D3/DRD3 antibody (ab42114)
Key features and details
- Rabbit polyclonal to Dopamine Receptor D3/DRD3
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Dopamine Receptor D3/DRD3 antibody
See all Dopamine Receptor D3/DRD3 primary antibodies -
Description
Rabbit polyclonal to Dopamine Receptor D3/DRD3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Rat
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Immunogen
Synthetic peptide corresponding to Dopamine Receptor D3/DRD3.
Sequence:CQACHVSPELYRATTWGY
(Peptide available asab128688) -
General notes
This product was previously labelled as Dopamine Receptor D3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Dopamine Receptor D3/DRD3 antibody (ab42114)Anti-Dopamine Receptor D3/DRD3 antibody (ab42114) at 1/750 dilution + PC-D3R sample
Predicted band size: 50 kDa
Observed band size: 50 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-Dopamine Receptor D3/DRD3 antibody (ab42114)ICC/IF image of ab42114 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42114, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
