Astana Biomed Group, an authorized Abcam distributor in Central Asia

PE Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] (ab252099)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • PE Rabbit monoclonal [EP2102Y] to cAMP Protein Kinase Catalytic subunit
  • Suitable for: Flow Cyt
  • Reacts with: Human
  • Conjugation: PE. Ex: 488nm, Em: 575nm

Overview

  • Product name

    PE Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y]
    See all cAMP Protein Kinase Catalytic subunit primary antibodies

  • Description

    PE Rabbit monoclonal [EP2102Y] to cAMP Protein Kinase Catalytic subunit

  • Host species

    Rabbit

  • Conjugation

    PE. Ex: 488nm, Em: 575nm

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt: MCF7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - PE Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] (ab252099)
    Flow Cytometry - PE Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] (ab252099)

    Overlay histogram showing MCF7 cells stained with ab252099 (red line). The cells were fixed with 80 % methanol (5 min) and then permeabilized with 0.1 % PBS-Triton X-100 for 15 min. The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab252099) (1x 106 in 100µl at 0.5 µg/ml (1/1000)) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in MCF7 cells fixed with 4 % formaldehyde (10 min) / permeabilized with 0.1 % PBS-Triton X-100 for 15 min used under the same conditions.

     

  • PE Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] (ab252099)
    PE Anti-cAMP Protein Kinase Catalytic subunit antibody [EP2102Y] (ab252099)

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