Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [E151] to PKA R2/PKR2 (phospho S99)
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human, Pig

Product name

Anti-PKA R2/PKR2 (phospho S99) antibody [E151]
See all PKA R2/PKR2 primary antibodies
Description

Rabbit monoclonal [E151] to PKA R2/PKR2 (phospho S99)
Host species

Rabbit
Specificity

The antibody only detects PKA R2/PKR2 phosphorylated on Serine 99. Phosphoserine 99 in Human corresponds to phosphoserine 96 in mouse.

The mouse and pig recommendation is based on the WB results. We do not guarantee IHC-P for mouse and pig.

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Rat Human
WB	Mouse Rat Human Pig

See all applications and species data

Immunogen

corresponding to Human PKA R2/PKR2 (phospho S99).
Positive control
- WB: K562 cell lysate. HeLa starved for 3 hours and then treated with 100 nM Calyculin A for 30 minutes, NIH/3T3, NIH/3T3 treated with Calyculin A, and C6 treated with 100 ng/ml Calyculin A for 1 hour cell lysates. Pig heart lysates. ICC/IF: HeLa cells. IHC-P: Human lymph node metastatic tissue. Human kidney without Lambda Protein Phosphatase treatment (image A) and Rat kidney without Lambda Protein Phosphatase treatment
General notes
This product was previously labelled as PKA R2

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

E151
Isotype

IgG
Research areas

Western blot - Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390)

All lanes : Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390) at 1/1000 dilution (Purified)

Lane 1 : Pig heart lysates
Lane 2 : Pig heart. Then the membrane was incubated with phosphatase

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 45 kDa
Observed band size: 51 kDa
why is the actual band size different from the predicted?
Western blot - Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390)

All lanes : Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390) at 1/1000 dilution (Purified)

Lane 1 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 2 : C6 (Rat glial tumor glial cell) treated with 100ng/ml Calyculin A for 1 hour
Lane 3 : C6 (Rat glial tumor glial cell) treated with 100ng/ml Calyculin A for 1 hour.Then the membrane was incubated with phosphatase

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 45 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Western blot - Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390)

All lanes : Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390) at 1/1000 dilution (Purified)

Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) treated with Calyculin A
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) treated with Calyculin A. Then the membrane was incubated with phosphatase

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 45 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Western blot - Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390)

All lanes : Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390) at 1/1000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) were starved for 3 hours and then treated with 100 nM Calyculin A for 30 minutes
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100 nM Calyculin A for 30 minutes. Then the membrane was incubated with phosphatase

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 45 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat kidney without Lambda Protein Phosphatase treatment (image A) and treated with Lambda Protein Phosphatase (image B). tissue sections labeling PKA R2/PKR2 with purified ab32390 at 1:500 dilution (0.75 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney without Lambda Protein Phosphatase treatment (image A) and treated with Lambda Protein Phosphatase (image B). tissue sections labeling PKA R2/PKR2 with purified ab32390 at 1:500 dilution (0.75 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390)

All lanes : Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390) at 1/2000 dilution

Lane 1 : K562 whole cell lysate with PKA R2/PKR2 (pS99) phospho peptide
Lane 2 : K562 whole cell lysate with PKA R2/PKR2 non-phospho peptide

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 45 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390)

Cell line: HeLa (human cervix adenocarcinoma)

Target AbID: Ab32390 anti-PKA R2/PKR2 (phospho S99)

Target Secondary: ab150077 AlexaFluor^®488 goat anti-rabbit

Counterstain AbID: ab7291 anti-Tubulin (mouse mAb)

Target Secondary: ab150120 AlexaFluor^®594 goat anti-mouse

Fixative: 4% PFA

Permeabilization: 0.1% Triton-X

Nuclear counter stain: DAPI

Other comments: Confocal image showed increased cytoplasmic staining after CA (100ng/ml, 10min) treatment on Hela cells. The LP treatment decreased the increased cytoplasmic staining caused by CA. Ab32514 was used as a Pan control for ab32390. The results showed cytoplasmic staining on untreated, CA and CA+LP treated Hela cells.
Western blot - Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390)

All lanes : Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390) at 1/10000 dilution

Lane 1 : Untreated K562 whole cell lysate
Lane 2 : K562 whole cell lysate - treated with alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 45 kDa
Observed band size: 51 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390)

ab32390, at a 1/250 dilution, staining (A) untreated (B) Phosphatase treated lymph node metastatic by immunohistochemistry, Paraffin embedded human tissue
Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKA R2/PKR2 (phospho S99) antibody [E151] (ab32390)

Key features and details

Overview

Product name

Description

Host species

Specificity

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Images

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Anti-PKA R2/PKR2 (phospho S96) antibody (ab226754)

Anti-PKA R2/PKR2 antibody [Y116] (ab32514)