Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (ab247490)
![Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (ab247490)](https://www.abcam.com/ps/products/247/ab247490/Images/ab247490-407937-anti-pias1-pias2-antibody-epr2581y-bsa-and-azide-free-western-blot.jpg "Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (ab247490)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2581Y] to PIAS1+PIAS2 - BSA and Azide free
- Suitable for: IHC-P, ICC, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free
See all PIAS1+PIAS2 primary antibodies -
Description
Rabbit monoclonal [EPR2581Y] to PIAS1+PIAS2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247490 is the carrier-free version of ab77231. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247490 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR2581Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (ab247490)All lanes : Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231) at 1/50000 dilution
Lane 1 : Human PIAS1 full-length recombinant protein with GST-Tag
Lane 2 : Human PIAS2-beta recombinant protein fragment with GST-His-Tag
Lane 3 : Human PIAS3 recombinant protein fragment with SUMO-His-Tag
Lysates/proteins at 0.015 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 72 kDa
Observed band size: 98, 42 kDa why is the actual band size different from the predicted?This data was developed using ab77231, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 10 seconds, Lane 2: 5 seconds, Lane 3: 3 minutes.
Human PIAS1 full-length recombinant protein (ab152888)
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![Western blot - Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (ab247490)](https://www.abcam.com/ps/products/247/ab247490/Images/ab247490-405583-anti-pias1-pias2-antibody-epr2581y-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (ab247490)All lanes : Anti-PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231) at 1/50000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : 293 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : SW40 cell lysate
Lane 5 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 72 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?This data was developed using ab77231, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry - Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (ab247490)](https://www.abcam.com/ps/products/247/ab247490/Images/ab247490-2-anti-pias1-pias2-antibody-epr2581y-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (ab247490)This data was developed using ab77231, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling PIAS1 + PIAS2 with purified ab77231 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue). Secondary Only Control: PBS was used instead of the primary antibody as the negative control. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (ab247490)](https://www.abcam.com/ps/products/247/ab247490/Images/ab247490-1-anti-pias1-pias2-antibody-epr2581y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (ab247490)This data was developed using ab77231, the same antibody clone in a different buffer formulation.ab77231, at a 1/100 dilution, staining PIAS1 + PIAS2 in paraffin embedded human tonsil tissue by Immunohistochemistry. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. -
![Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (ab247490)](https://www.abcam.com/ps/products/247/ab247490/Images/ab247490-5-benefits-of-recombinant-antibodies.png)
Anti-PIAS1+PIAS2 antibody [EPR2581Y] - BSA and Azide free (ab247490)