Anti-PIAS3 antibody (ab105178)
Key features and details
- Rabbit polyclonal to PIAS3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PIAS3 antibody
See all PIAS3 primary antibodies -
Description
Rabbit polyclonal to PIAS3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human PIAS3 aa 550 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab123057) -
Positive control
- This antibody gave a positive signal in both HepG2 and Caco2 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseHorseCowDogPigMacaque monkeyGorillaApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 68 kDa).Target
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Function
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation (PubMed:21965678, PubMed:9388184). Sumoylates CCAR2 which promotes its interaction with SIRT1 (PubMed:25406032). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (PubMed:24651376). -
Tissue specificity
Widely expressed. -
Pathway
Protein modification; protein sumoylation. -
Sequence similarities
Belongs to the PIAS family.
Contains 1 PINIT domain.
Contains 1 SAP domain.
Contains 1 SP-RING-type zinc finger. -
Domain
The PINIT domain of PIAS3 is required for STAT3-PIAS3 interaction and for transloaction to the nucleus.
The LXXLL motif is a transcriptional coregulator signature. -
Post-translational
modificationsSumoylated. -
Cellular localization
Cytoplasm. Nucleus. Nucleus speckle. Colocalizes with MITF in the nucleus. Colocalizes with GFI1 in nuclear dots. Colocalizes with SUMO1 in nuclear granules. - Information by UniProt
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Database links
- Entrez Gene: 10401 Human
- Entrez Gene: 229615 Mouse
- Omim: 605987 Human
- SwissProt: Q9Y6X2 Human
- SwissProt: O54714 Mouse
- Unigene: 435761 Human
- Unigene: 1635 Mouse
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Alternative names
- E3 SUMO protein ligase PIAS 3 antibody
- E3 SUMO protein ligase PIAS3 antibody
- E3 SUMO-protein ligase PIAS3 antibody
see all
Images
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Western blot - Anti-PIAS3 antibody (ab105178)All lanes : Anti-PIAS3 antibody (ab105178) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
PIAS3 contains a potential sumoylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
References (0)
ab105178 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-PIAS3 antibody (ab105178)All lanes : Anti-PIAS3 antibody (ab105178) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
Additional bands at: 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
PIAS3 contains a potential sumoylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
