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Anti-Phosphotyrosine antibody [RM111] (ab190824)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM111] to Phosphotyrosine
  • Suitable for: WB, IP, ELISA, IHC-P, Flow Cyt, ChIP, ICC/IF
  • Reacts with: Species independent

Overview

  • Product name

    Anti-Phosphotyrosine antibody [RM111]
    See all Phosphotyrosine primary antibodies

  • Description

    Rabbit monoclonal [RM111] to Phosphotyrosine

  • Host species

    Rabbit

  • Specificity

    This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with non phosphorylated tyrosine, phospho-serine, and phospho-threonine

  • Tested applications

    Suitable for: WB, IP, ELISA, IHC-P, Flow Cyt, ChIP, ICC/IFmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Chemical/ Small Molecule corresponding to Phosphotyrosine conjugated to Bovine Serum Albumin (BSA).

  • Positive control

    • Serum starved, EGF treated A431 cells; serum starved, EGF treated A431 cell lysate; EGF treated, A431 cell lysate.

Properties

Images

  • Western blot - Anti-Phosphotyrosine antibody [RM111] (ab190824)
    Western blot - Anti-Phosphotyrosine antibody [RM111] (ab190824)
    All lanes : Anti-Phosphotyrosine antibody [RM111] (ab190824) at 1/5000 dilution

    Lanes 1 & 3 & 5 : Non treated serum-starved A431 cell lysate
    Lanes 2 & 4 & 6 : EGF treated serum-starved A431 cell lysate


    The blot was was subjected twice to increasing exposure time in Lanes 3 and 4, and Lanes 5 and 6.

  • Immunoprecipitation - Anti-Phosphotyrosine antibody [RM111] (ab190824)
    Immunoprecipitation - Anti-Phosphotyrosine antibody [RM111] (ab190824)

    Immunoprecipitation of EGF-treated A431 cells pulling down Phosphotyrosine-EGFR by ab190824 at 1/1000 dilution; this was followed by western blot with an anti-EGFR rabbit monoclonal antibody.
    (1) Whole lysate control;

    (2) IP by rabbit IgG control;

    (3) IP by ab190824

  • Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [RM111] (ab190824)
    Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [RM111] (ab190824)

    Immunocytochemistry of serum-starved A431 cells nontreated (Left) or treated with EGF (Right) labeling Phosphotyrosine-EGFR using ab190824 at 1/500 dilution  (followed by a PE conjugated secondary antibody, red) and DAPI (blue).

  • ELISA - Anti-Phosphotyrosine antibody [RM111] (ab190824)
    ELISA - Anti-Phosphotyrosine antibody [RM111] (ab190824)

    Analysis of ab190824 reaction to phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. ab190824 only reacted to phosphorylated Tyrosine.

  • Anti-Phosphotyrosine antibody [RM111] (ab190824)
    Anti-Phosphotyrosine antibody [RM111] (ab190824)

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