Anti-Phosphotyrosine antibody [RM111] (ab190824)
![Anti-Phosphotyrosine antibody [RM111] (ab190824)](https://www.abcam.com/ps/products/190/ab190824/Images/ab190824-226568-anti-phosphotyrosine-antibody-rm111-western-blot.jpg "Anti-Phosphotyrosine antibody [RM111] (ab190824)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM111] to Phosphotyrosine
- Suitable for: WB, IP, ELISA, IHC-P, Flow Cyt, ChIP, ICC/IF
- Reacts with: Species independent
Overview
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Product name
Anti-Phosphotyrosine antibody [RM111]
See all Phosphotyrosine primary antibodies -
Description
Rabbit monoclonal [RM111] to Phosphotyrosine -
Host species
Rabbit -
Specificity
This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with non phosphorylated tyrosine, phospho-serine, and phospho-threonine -
Tested applications
Suitable for: WB, IP, ELISA, IHC-P, Flow Cyt, ChIP, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Phosphotyrosine conjugated to Bovine Serum Albumin (BSA).
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Positive control
- Serum starved, EGF treated A431 cells; serum starved, EGF treated A431 cell lysate; EGF treated, A431 cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM111 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Phosphotyrosine antibody [RM111] (ab190824)All lanes : Anti-Phosphotyrosine antibody [RM111] (ab190824) at 1/5000 dilution
Lanes 1 & 3 & 5 : Non treated serum-starved A431 cell lysate
Lanes 2 & 4 & 6 : EGF treated serum-starved A431 cell lysateThe blot was was subjected twice to increasing exposure time in Lanes 3 and 4, and Lanes 5 and 6.
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![Immunoprecipitation - Anti-Phosphotyrosine antibody [RM111] (ab190824)](https://www.abcam.com/ps/products/190/ab190824/Images/ab190824-226566-anti-phosphotyrosine-antibody-rm111-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Phosphotyrosine antibody [RM111] (ab190824)Immunoprecipitation of EGF-treated A431 cells pulling down Phosphotyrosine-EGFR by ab190824 at 1/1000 dilution; this was followed by western blot with an anti-EGFR rabbit monoclonal antibody.
(1) Whole lysate control;(2) IP by rabbit IgG control;
(3) IP by ab190824
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![Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [RM111] (ab190824)](https://www.abcam.com/ps/products/190/ab190824/Images/ab190824-226564-anti-phosphotyrosine-antibody-rm111-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Phosphotyrosine antibody [RM111] (ab190824)Immunocytochemistry of serum-starved A431 cells nontreated (Left) or treated with EGF (Right) labeling Phosphotyrosine-EGFR using ab190824 at 1/500 dilution (followed by a PE conjugated secondary antibody, red) and DAPI (blue).
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![ELISA - Anti-Phosphotyrosine antibody [RM111] (ab190824)](https://www.abcam.com/ps/products/190/ab190824/Images/ab190824-226563-anti-phosphotyrosine-antibody-rm111-elisa.jpg)
ELISA - Anti-Phosphotyrosine antibody [RM111] (ab190824)Analysis of ab190824 reaction to phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. ab190824 only reacted to phosphorylated Tyrosine.
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![Anti-Phosphotyrosine antibody [RM111] (ab190824)](https://www.abcam.com/ps/products/190/ab190824/Images/ab190824-5-benefits-of-recombinant-antibodies.png)
Anti-Phosphotyrosine antibody [RM111] (ab190824)
