Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Peroxiredoxin 2/PRP antibody [EPR5155] - BSA and Azide free (ab248516)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5155] to Peroxiredoxin 2/PRP - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Peroxiredoxin 2/PRP antibody [EPR5155] - BSA and Azide free
    See all Peroxiredoxin 2/PRP primary antibodies

  • Description

    Rabbit monoclonal [EPR5155] to Peroxiredoxin 2/PRP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, Flow Cytmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293T, HepG2, LnCaP and U937 cell lysates. ICC: HeLa cells. Flow Cyt: HeLa cells.

  • General notes

    Ab248516 is the carrier-free version of ab133481. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248516 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] - BSA and Azide free (ab248516)
    Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] - BSA and Azide free (ab248516)

    This data was developed using ab133481, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (20 µg)

    Lane 2: Peroxiredoxin 2/PRP knockout HAP1 cell lysate (20 µg)

    Lane 3: HeLa cell lysate (20 µg)

    Lane 4: LnCaP cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab133481 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab133481 was shown to specifically react with Peroxiredoxin 2/PRP when Peroxiredoxin 2/PRP knockout samples were used. Wild-type and Peroxiredoxin 2/PRP knockout samples were subjected to SDS-PAGE. ab133481 and ab8245 (loading control to GAPDH) were diluted 1/50 000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)

    and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Flow Cytometry - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] - BSA and Azide free (ab248516)
    Flow Cytometry - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] - BSA and Azide free (ab248516)
    This data was developed using ab133481, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab133481 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133481, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] - BSA and Azide free (ab248516)
    Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] - BSA and Azide free (ab248516)
    All lanes : Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (ab133481) at 1/5000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : PRDX2 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab133481).

    Lanes 1- 2: Merged signal (red and green). Green - ab133481 observed at 22 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab133481 was shown to react with Peroxiredoxin 2/PRP in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266392 (knockout cell lysate ab257041) was used. Wild-type HEK-293T and PRDX2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133481 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] - BSA and Azide free (ab248516)
    Western blot - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] - BSA and Azide free (ab248516)
    All lanes : Anti-Peroxiredoxin 2/PRP antibody [EPR5155] (ab133481) at 1/50000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : LnCaP cell lysate
    Lane 4 : U937 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 22 kDa



    This data was developed using ab133481, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] - BSA and Azide free (ab248516)
    Immunocytochemistry - Anti-Peroxiredoxin 2/PRP antibody [EPR5155] - BSA and Azide free (ab248516)
    This data was developed using ab133481, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of HeLa cells labelling Peroxiredoxin 2/PRP with ab133481 at 1/100 dilution.
  • Anti-Peroxiredoxin 2/PRP antibody [EPR5155] - BSA and Azide free (ab248516)
    Anti-Peroxiredoxin 2/PRP antibody [EPR5155] - BSA and Azide free (ab248516)

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