Anti-Peroxiredoxin 1/PAG antibody [3G5] (ab70666)
![Anti-Peroxiredoxin 1/PAG antibody [3G5] (ab70666)](https://www.abcam.com/ps/products/70/ab70666/Images/ab70666-260944-ab70666KOWB.jpg "Anti-Peroxiredoxin 1/PAG antibody [3G5] (ab70666)")
Key features and details
- Mouse monoclonal [3G5] to Peroxiredoxin 1/PAG
- Suitable for: IHC-P, WB, IP
- Knockout validated
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
-
Product name
Anti-Peroxiredoxin 1/PAG antibody [3G5]
See all Peroxiredoxin 1/PAG primary antibodies -
Description
Mouse monoclonal [3G5] to Peroxiredoxin 1/PAG -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP HumanWB HumanRecombinant fragment
-
Immunogen
Recombinant full length protein corresponding to Human Peroxiredoxin 1/PAG.
-
Positive control
- WB: HeLa, A431, HL-60 and SK-N-MC cell lysates. IHC-P: Human thyroid tissue. IP: HeLa cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.03% Sodium azide
Constituents: HEPES, 0.01% BSA, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3G5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
-
Western blot - Anti-Peroxiredoxin 1/PAG antibody [3G5] (ab70666)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Peroxiredoxin 1/PAG knockout HAP1 cell lysate (20 µg)
Lane 3: A431 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab70666 observed at 23 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab70666 was shown to specifically react with Peroxiredoxin 1/PAG when Peroxiredoxin 1//PAG knockout samples were used. Wild-type and Peroxiredoxin 1//PAG knockout samples were subjected to SDS-PAGE. ab70666 and ab181602 (loading control to GAPDH) were diluted 1/20 000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-Peroxiredoxin 1/PAG antibody [3G5] (ab70666)](https://www.abcam.com/ps/products/70/ab70666/Images/ab70666-288894-ab70666wb.jpg)
Western blot - Anti-Peroxiredoxin 1/PAG antibody [3G5] (ab70666)All lanes : Anti-Peroxiredoxin 1/PAG antibody [3G5] (ab70666) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SK-N-MC cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HL60 cell lysate
Predicted band size: 22 kDaIncubated with the primary antibody overnight at 4°C. Incubated with the secondary antibody for 1 hour at room temperature.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody [3G5] (ab70666)](https://www.abcam.com/ps/products/70/ab70666/Images/ab70666-288922-ab70666ihcp.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Peroxiredoxin 1/PAG antibody [3G5] (ab70666)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid tissue labelling Peroxiredoxin 1/PAG with ab70666.
-
![Immunoprecipitation - Anti-Peroxiredoxin 1/PAG antibody [3G5] (ab70666)](https://www.abcam.com/ps/products/70/ab70666/Images/ab70666-288896-ab70666ip.jpg)
Immunoprecipitation - Anti-Peroxiredoxin 1/PAG antibody [3G5] (ab70666)Immunoprecipitation analysis using ab70666.
Lane 1: HeLa cell lysate
Lane 2: Immunoprecipitate from HeLa cell lysate (200 µg) using ab70666 (1 µg)
Lane 3: Immunoprecipitate from HeLa cell lysate (200 µg) using ab70666 (2 µg)
Lane 4: Immunoprecipitate from PBS using ab70666 (2 µg)ab70666 was also used for western blot at 1/20000 overnight at 4°C.
Incubated with the secondary antibody at 1/5000 for 1 hour at room temperature.
-
![Western blot - Anti-Peroxiredoxin 1/PAG antibody [3G5] (ab70666)](https://www.abcam.com/ps/products/70/ab70666/Images/ab70666-288895-ab70666wb2.jpg)
Western blot - Anti-Peroxiredoxin 1/PAG antibody [3G5] (ab70666)All lanes : Anti-Peroxiredoxin 1/PAG antibody [3G5] (ab70666) at 1/20000 dilution
Lane 1 : Peroxiredoxin 1/PAG recombinant protein
Lane 2 : Peroxiredoxin 2/PAG recombinant protein
Lane 3 : Peroxiredoxin 3/PAG recombinant protein
Lane 4 : Peroxiredoxin 4/PAG recombinant protein
Lane 5 : Peroxiredoxin 5/PAG recombinant protein
Lane 6 : Peroxiredoxin 6/PAG recombinant protein
Predicted band size: 22 kDaIncubated with the primary antibody overnight at 4°C. Incubated with the secondary antibody for 1 hour at room temperature.
