Anti-Prokineticin 2/PK2 antibody (ab87360)
Key features and details
- Rabbit polyclonal to Prokineticin 2/PK2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Prokineticin 2/PK2 antibody
See all Prokineticin 2/PK2 primary antibodies -
Description
Rabbit polyclonal to Prokineticin 2/PK2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Human Prokineticin 2/PK2 aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab123828) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Human Testis; Human Small Intestine; Mouse Testis; Rat Testis.
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General notes
This product was previously labelled as Prokineticin 2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87360 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications Macaque monkeyGorillaApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 14 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 14 kDa).Target
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Function
May function as an output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm. May also function locally within the SCN to synchronize output. Potently contracts gastrointestinal (GI) smooth muscle. -
Tissue specificity
Expressed in the testis and, at low levels, in the small intestine. -
Involvement in disease
Defects in PROK2 are the cause of Kallmann syndrome type 4 (KAL4) [MIM:610628]; also known as hypogonadotropic hypogonadism and anosmia. Anosmia or hyposmia is related to the absence or hypoplasia of the olfactory bulbs and tracts. Hypogonadism is due to deficiency in gonadotropin-releasing hormone and probably results from a failure of embryonic migration of gonadotropin-releasing hormone-synthesizing neurons. KAL4 patients have variable degrees of olfactory and reproductive dysfunction, but do not show any of the occasional clinical anomalies reported in Kallmann syndrome such as renal agenesis, cleft lip/palate, selective tooth agenesis, and bimanual synkinesis. -
Sequence similarities
Belongs to the AVIT (prokineticin) family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 101143069 Gorilla
- Entrez Gene: 60675 Human
- Entrez Gene: 50501 Mouse
- Entrez Gene: 192206 Rat
- Omim: 607002 Human
- SwissProt: Q9HC23 Human
- SwissProt: Q9QXU7 Mouse
- SwissProt: Q8R413 Rat
see all -
Alternative names
- BV8 antibody
- Bv8 homolog antibody
- MIT1 antibody
see all
Images
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Western blot - Anti-Prokineticin 2/PK2 antibody (ab87360)All lanes : Anti-Prokineticin 2/PK2 antibody (ab87360) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lane 3 : Testis (Mouse) Tissue Lysate
Lane 4 : Testis (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 115 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Datasheets and documents
References (1)
ab87360 has been referenced in 1 publication.
- Zhang Y et al. MicroRNA-374a Governs Aggressive Cell Behaviors of Glioma by Targeting Prokineticin 2. Technol Cancer Res Treat 18:1533033818821401 (2019). PubMed: 30803356
Images
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Western blot - Anti-Prokineticin 2/PK2 antibody (ab87360)All lanes : Anti-Prokineticin 2/PK2 antibody (ab87360) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lane 3 : Testis (Mouse) Tissue Lysate
Lane 4 : Testis (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
Additional bands at: 115 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
