Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free (ab251492)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19727] to PDK1 + PDK2 + PDK4 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free
    See all PDK1 + PDK2 + PDK4 primary antibodies

  • Description

    Rabbit monoclonal [EPR19727] to PDK1 + PDK2 + PDK4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251492 is the carrier-free version of ab208187. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251492 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR19727

  • Isotype

    IgG

Images

  • Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free (ab251492)
    Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free (ab251492)
    All lanes : Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187) at 1/10000 dilution

    Lane 1 : Mouse PDK4 recombinant protein
    Lane 2 : Mouse PDK1 recombinant protein
    Lane 3 : Mouse PDK2 recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa


    Exposure time: 1 second


    This data was developed using ab208187, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Mouse PDK4, PDK1 and PDK2 recombinant proteins contain aa8-412, aa1-434 and aa1-407 respectively with a His-Tag®.

  • Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free (ab251492)
    Western blot - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free (ab251492)
    All lanes : Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] (ab208187) at 1/1000 dilution

    Lane 1 : Mouse skeletal muscle lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse liver tissue lysate
    Lane 4 : C2C12 (Mouse myoblast cell line) whole cell lysate
    Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 6 : Human heart lysate
    Lane 7 : Rat heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 47 kDa
    Observed band size: 47 kDa



    This data was developed using ab208187, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-4: 10 seconds; Lanes 5-6:3 minutes; Lane 7: 2 seconds.

  • Immunoprecipitation - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free (ab251492)
    Immunoprecipitation - Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free (ab251492)
    This data was developed using ab208187, the same antibody clone in a different buffer formulation.PDK1+PDK2+PDK4 was immunoprecipitated from 0.35 mg of Mouse skeletal muscle whole cell lysate with ab208187 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab208187 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution. Lane 1: Mouse skeletal muscle whole cell lysate 10µg (Input). Lane 2: ab208187 IP in Mouse skeletal muscle whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208187 in Mouse skeletal muscle whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 seconds.
  • Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free (ab251492)
    Anti-PDK1 + PDK2 + PDK4 antibody [EPR19727] - BSA and Azide free (ab251492)

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