Anti-PDE4B antibody [EPR11830] - BSA and Azide free (ab249608)
![Anti-PDE4B antibody [EPR11830] - BSA and Azide free (ab249608)](https://www.abcam.com/ps/products/249/ab249608/Images/ab249608-393712-anti-pde4b-antibody-epr11830-western-blot-human-knockout-lysate.jpg "Anti-PDE4B antibody [EPR11830] - BSA and Azide free (ab249608)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11830] to PDE4B - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Knockout validated
- Reacts with: Mouse, Human
Overview
-
Product name
Anti-PDE4B antibody [EPR11830] - BSA and Azide free
See all PDE4B primary antibodies -
Description
Rabbit monoclonal [EPR11830] to PDE4B - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Recombinant fragment within Human PDE4B aa 550 to the C-terminus. The exact sequence is proprietary.
Database link: Q07343 -
Positive control
- WB: HeLa and A549 cell lysates; Mouse brain lysate.
-
General notes
Ab249608 is the carrier-free version of ab170939. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249608 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11830 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-PDE4B antibody [EPR11830] - BSA and Azide free (ab249608)All lanes : Anti-PDE4B antibody [EPR11830] (ab170939) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PDE4B knockout HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Mouse brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab170939).
Lanes 1-4: Merged signal (red and green). Green - ab170939 observed at 83 kDa. Red - loading control ab8245 observed at 36 kDa.
ab170939 Anti-PDE4B antibody [EPR11830] was shown to specifically react with PDE4B in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265259 (knockout cell lysate ab257576) was used. Wild-type and PDE4B knockout samples were subjected to SDS-PAGE. ab170939 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Anti-PDE4B antibody [EPR11830] - BSA and Azide free (ab249608)](https://www.abcam.com/ps/products/249/ab249608/Images/ab249608-2-benefits-of-recombinant-antibodies.png)
Anti-PDE4B antibody [EPR11830] - BSA and Azide free (ab249608)
