Anti-PDCD6/ALG-2 antibody [EP3259] - BSA and Azide free (ab240941)
![Anti-PDCD6/ALG-2 antibody [EP3259] - BSA and Azide free (ab240941)](https://www.abcam.com/ps/products/240/ab240941/Images/ab240941-397715-anti-pdcd6alg-2-antibody-ep3259-western-blot-human-knockout-lysate.jpg "Anti-PDCD6/ALG-2 antibody [EP3259] - BSA and Azide free (ab240941)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3259] to PDCD6/ALG-2 - BSA and Azide free
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PDCD6/ALG-2 antibody [EP3259] - BSA and Azide free
See all PDCD6/ALG-2 primary antibodies -
Description
Rabbit monoclonal [EP3259] to PDCD6/ALG-2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human hepatoma and Human lung adenocarcinoma. WB: HEK293T, HeLa and Daudi cell lysates.
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General notes
ab240941 is the carrier-free version of ab109181 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab240941 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Previously labeled as PDCD6.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP3259 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PDCD6/ALG-2 antibody [EP3259] - BSA and Azide free (ab240941)All lanes : Anti-PDCD6/ALG-2 antibody [EP3259] (ab109181) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : PDCD6 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab109181).
Lanes 1-4: Merged signal (red and green). Green - ab109181 observed at 22 kDa. Red - loading control ab8245 observed at 36 kDa.
ab109181 Anti-PDCD6/ALG-2 antibody [EP3259] was shown to specifically react with PDCD6/ALG-2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266323 (knockout cell lysate ab258570) was used. Wild-type and PDCD6/ALG-2 knockout samples were subjected to SDS-PAGE. ab109181 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD6/ALG-2 antibody [EP3259] - BSA and Azide free (ab240941)](https://www.abcam.com/ps/products/240/ab240941/Images/ab240941-1-PDCD6Primaryantibodiesab1091812.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD6/ALG-2 antibody [EP3259] - BSA and Azide free (ab240941)Immunohistochemical analysis of paraffin-embedded Human hepatoma using ab109181 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109181).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD6/ALG-2 antibody [EP3259] - BSA and Azide free (ab240941)](https://www.abcam.com/ps/products/240/ab240941/Images/ab240941-2-PDCD6Primaryantibodiesab1091813.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD6/ALG-2 antibody [EP3259] - BSA and Azide free (ab240941)Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma using ab109181 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109181).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-PDCD6/ALG-2 antibody [EP3259] - BSA and Azide free (ab240941)](https://www.abcam.com/ps/products/240/ab240941/Images/ab240941-4-benefits-of-recombinant-antibodies.png)
Anti-PDCD6/ALG-2 antibody [EP3259] - BSA and Azide free (ab240941)
