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Anti-TRC40 antibody [EPR11423] (ab169539)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11423] to TRC40
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-TRC40 antibody [EPR11423]
    See all TRC40 primary antibodies

  • Description

    Rabbit monoclonal [EPR11423] to TRC40

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human TRC40 aa 300-400. The exact sequence is proprietary.
    Database link: O43681

  • Positive control

    • Fetal brain, fetal kidney, HeLa and 293T lysates. Human colon and Human lung tissue. HeLa and permeabilized 293T cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR11423

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TRC40 antibody [EPR11423] (ab169539)
    Western blot - Anti-TRC40 antibody [EPR11423] (ab169539)
    All lanes : Anti-TRC40 antibody [EPR11423] (ab169539) at 1/10000 dilution

    Lane 1 : Fetal brain lysate
    Lane 2 : Fetal kidney lysate
    Lane 3 : HeLa lysate
    Lane 4 : 293T lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 39 kDa
    Observed band size: 39 kDa

  • Immunoprecipitation - Anti-TRC40 antibody [EPR11423] (ab169539)
    Immunoprecipitation - Anti-TRC40 antibody [EPR11423] (ab169539)

    Immunoprecipitation. ab169539 at 1/10000 staining TRC40 in 293T cell lysate immunoprecipitated using ab169539 at 1/10. Predicted band size : 39 kDa.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRC40 antibody [EPR11423] (ab169539)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRC40 antibody [EPR11423] (ab169539)

    Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TRC40 with ab169539 antibody at 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRC40 antibody [EPR11423] (ab169539)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRC40 antibody [EPR11423] (ab169539)

    Immunohistochemical analysis of paraffin embedded Human lung tissue labeling TRC40 with ab169539 antibody at 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TRC40 antibody [EPR11423] (ab169539)
    Immunocytochemistry/ Immunofluorescence - Anti-TRC40 antibody [EPR11423] (ab169539)

    Immunofluorescent analysis of HeLa cells labeling TRC40 with ab169539 at 1/250.

  • Flow Cytometry - Anti-TRC40 antibody [EPR11423] (ab169539)
    Flow Cytometry - Anti-TRC40 antibody [EPR11423] (ab169539)
    Flow Cytometrical analysis of permeabilized 293T cells with ab169539 antibody at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
  • Anti-TRC40 antibody [EPR11423] (ab169539)
    Anti-TRC40 antibody [EPR11423] (ab169539)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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