Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCGF5 antibody [EP19476] - BSA and Azide free (ab251349)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP19476] to PCGF5 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PCGF5 antibody [EP19476] - BSA and Azide free
    See all PCGF5 primary antibodies

  • Description

    Rabbit monoclonal [EP19476] to PCGF5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251349 is the carrier-free version of ab201511. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251349 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PCGF5 antibody [EP19476] - BSA and Azide free (ab251349)
    Western blot - Anti-PCGF5 antibody [EP19476] - BSA and Azide free (ab251349)
    All lanes : Anti-PCGF5 antibody [EP19476] (ab201511) at 1/1000 dilution

    Lane 1 : Mouse testis lysate
    Lane 2 : Mouse ovary lysate
    Lane 3 : Mouse thymus lysate
    Lane 4 : mES-D3 (Mouse embryo stem cell line) whole cell lysate
    Lane 5 : F9 (Mouse embryonic testicular cancer cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa


    Exposure time: 3 minutes


    This data was developed using ab201511, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PCGF5 antibody [EP19476] - BSA and Azide free (ab251349)
    Western blot - Anti-PCGF5 antibody [EP19476] - BSA and Azide free (ab251349)
    All lanes : Anti-PCGF5 antibody [EP19476] (ab201511) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 5 : Rat testis lysate
    Lane 6 : Human testis lysate
    Lane 7 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 30 kDa
    Observed band size: 30 kDa



    This data was developed using ab201511, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-4: 30 seconds; Lanes 5-7: 3 minutes.

  • Anti-PCGF5 antibody [EP19476] - BSA and Azide free (ab251349)
    Anti-PCGF5 antibody [EP19476] - BSA and Azide free (ab251349)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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