Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PAX6 antibody [AD2.35] (ab245110)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [AD2.35] to PAX6
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PAX6 antibody [AD2.35]
    See all PAX6 primary antibodies

  • Description

    Mouse monoclonal [AD2.35] to PAX6

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human PAX6 aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P26367

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human, mouse and rat retina tissue. Human pancreas tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.35] (ab245110)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.35] (ab245110)

    Immunohistochemical analysis of paraffin-embedded human retina tissue labeling PAX6 with ab245110 at 4.13µg/ml, followed by a ready to use secondary. Nuclear staining on human retina is observed. The section was incubated with ab245110 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.35] (ab245110)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.35] (ab245110)

    Immunohistochemical analysis of paraffin-embedded mouse retina tissue labeling PAX6 with ab245110 at 8.26µg/ml, followed by a ready to use secondary. Nuclear staining on mouse retina is observed. The section was incubated with ab245110 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.


    Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.35] (ab245110)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.35] (ab245110)

    Immunohistochemical analysis of paraffin-embedded rat retina tissue labeling PAX6 with ab245110 at 8.26µg/ml, followed by a ready to use secondary. Nuclear staining on rat retina is observed. The section was incubated with ab245110 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by a ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.35] (ab245110)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.35] (ab245110)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling PAX6 with ab245110 at 4.13µg/ml followed by a ready to use secondary. Nuclear staining on human pancreas is observed. The section was incubated with ab245110 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Anti-PAX6 antibody [AD2.35] (ab245110)
    Anti-PAX6 antibody [AD2.35] (ab245110)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PAX6 antibody [AD2.35] (ab245110)

© 2021 Astana Biomed Group LLP. All rights reserved.