Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PAX6 antibody [AD2.35] - BSA and Azide free (ab255758)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [AD2.35] to PAX6 - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PAX6 antibody [AD2.35] - BSA and Azide free
    See all PAX6 primary antibodies

  • Description

    Mouse monoclonal [AD2.35] to PAX6 - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human PAX6 aa 1-250. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P26367

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human, mouse and rat retina tissue. Human pancreas tissue.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255758 is the carrier-free version of ab245110. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.35] - BSA and Azide free (ab255758)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.35] - BSA and Azide free (ab255758)

    Immunohistochemical analysis of paraffin-embedded human retina tissue labeling PAX6 with ab245110 at 4.13µg/ml, followed by a ready to use secondary. Nuclear staining on human retina is observed. The section was incubated with ab245110 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation ab245110, PBS, sodium azide, glycerol and BSA.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.35] - BSA and Azide free (ab255758)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.35] - BSA and Azide free (ab255758)

    Immunohistochemical analysis of paraffin-embedded mouse retina tissue labeling PAX6 with ab245110 at 8.26µg/ml followed by a ready to use secondary. Nuclear staining on mouse retina is observed. The section was incubated with ab245110 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation ab245110, PBS, sodium azide, glycerol and BSA.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.35] - BSA and Azide free (ab255758)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.35] - BSA and Azide free (ab255758)

    Immunohistochemical analysis of paraffin-embedded rat retina tissue labeling PAX6 with ab245110 at 8.26µg/ml, followed by a ready to use secondary. Nuclear staining on rat retina is observed. The section was incubated with ab245110 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation ab245110, PBS, sodium azide, glycerol and BSA.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.35] - BSA and Azide free (ab255758)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [AD2.35] - BSA and Azide free (ab255758)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling PAX6 with ab245110 at 4.13µg/ml, followed by a ready to use secondary. Nuclear staining on human pancreas is observed. The section was incubated with ab245110 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use secondary.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    This image was produced using the same antibody clone but in a different formulation ab245110, PBS, sodium azide, glycerol and BSA.

  • Anti-PAX6 antibody [AD2.35] - BSA and Azide free (ab255758)
    Anti-PAX6 antibody [AD2.35] - BSA and Azide free (ab255758)

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