Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free (ab243695)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13091] to Parvalbumin - BSA and Azide free
  • Suitable for: IHC-P, IP, IHC-Fr, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free
    See all Parvalbumin primary antibodies

  • Description

    Rabbit monoclonal [EPR13091] to Parvalbumin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, IHC-Fr, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: human kidney carcinoma, and human cerebrum tissues. Human chromophobe carcinoma tissue IHC-Fr: Mouse and rat cerebellum. WB: Human cerebellum tissue lysate; Recombinant Human Parvalbumin protein (ab101107) can be used as a positive control in WB.

  • General notes

    ab243695 is the carrier-free version of ab181086.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free (ab243695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free (ab243695)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Parvalbumin with purified ab181086 at 1/450 dilution (0.46 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181086).

  • Immunohistochemistry (Frozen sections) - Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free (ab243695)
    Immunohistochemistry (Frozen sections) - Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free (ab243695)

    ab181086 staining Parvalbumin in rat cerebrum tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA, permeabilized with 0.2% Triton X-100. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Samples were incubated with primary antibody (1/250). ab150077 an AlexaFluor®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. The Nuclear counter stain DAPI was used.

    Positive staining on purkinje cells and neuron cells of rat cerebellum.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181086).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free (ab243695)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free (ab243695)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney carcinoma tissue sections labeling Parvalbumin with purified ab181086 at 1/450 dilution (0.46 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181086)

  • Immunohistochemistry (Frozen sections) - Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free (ab243695)
    Immunohistochemistry (Frozen sections) - Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free (ab243695)

    Unpurified ab181086 staining Parvalbumin in Mouse cerebrum tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA, permeabilized with 0.2% Triton. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Samples were incubated with primary antibody (1/100). Ab150077 an AlexaFluor®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. The Nuclear counter stain DAPI was used. 

    Positive staining on Purkinje cells and neurons of the molecular layer in cerebellum (PMID: 22561329).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181086).

  • Immunohistochemistry (Frozen sections) - Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free (ab243695)
    Immunohistochemistry (Frozen sections) - Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free (ab243695)

    Unpurified ab181086 staining Parvalbumin in Mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA, permeabilized with 0.2% Triton. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Samples were incubated with primary antibody (1/100). Ab150077 an AlexaFluor®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. The Nuclear counter stain DAPI was used. 

    Positive staining on skeletal muscles (PMID: 7604022).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181086).

  • Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free (ab243695)
    Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free (ab243695)

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