Anti-PARP1 antibody [E78] - BSA and Azide free (ab247222)
![Anti-PARP1 antibody [E78] - BSA and Azide free (ab247222)](https://www.abcam.com/ps/products/247/ab247222/Images/ab247222-406313-anti-parp1-antibody-e78-bsa-and-azide-free-western-blot.jpg "Anti-PARP1 antibody [E78] - BSA and Azide free (ab247222)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E78] to PARP1 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-PARP1 antibody [E78] - BSA and Azide free
See all PARP1 primary antibodies -
Description
Rabbit monoclonal [E78] to PARP1 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody should recognise both pro-form and p85 cleaved-form of PARP1.
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Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247222 is the carrier-free version of ab32071 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247222 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E78 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PARP1 antibody [E78] - BSA and Azide free (ab247222)
This data was developed using ab32071, the same antibody clone in a different buffer formulation.
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: PARP1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: MCF7 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green ab32071 observed at 125 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32071 was shown to specifically react with PARP1 when PARP1 knockout samples were used. Wild-type and PARP1 knockout samples were subjected to SDS-PAGE. ab32071 and ab8245 (Mouse anti- GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10 000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PARP1 antibody [E78] - BSA and Azide free (ab247222)](https://www.abcam.com/ps/products/247/ab247222/Images/ab247222-397857-anti-parp1-antibody-e78-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-PARP1 antibody [E78] - BSA and Azide free (ab247222) -
![Anti-PARP1 antibody [E78] - BSA and Azide free (ab247222)](https://www.abcam.com/ps/products/247/ab247222/Images/ab247222-3-benefits-of-recombinant-antibodies.png)
Anti-PARP1 antibody [E78] - BSA and Azide free (ab247222)