Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PAR4 antibody [EPR3991] - BSA and Azide free (ab247588)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3991] to PAR4 - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-PAR4 antibody [EPR3991] - BSA and Azide free
    See all PAR4 primary antibodies

  • Description

    Rabbit monoclonal [EPR3991] to PAR4 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247588 is the carrier-free version of ab92590 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247588 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PAR4 antibody [EPR3991] - BSA and Azide free (ab247588)
    Western blot - Anti-PAR4 antibody [EPR3991] - BSA and Azide free (ab247588)
    All lanes : Anti-PAR4 antibody [EPR3991] (ab92590) at 1/10000 dilution

    Lane 1 : LNCap cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 37 kDa



    This data was developed using ab92590, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-PAR4 antibody [EPR3991] - BSA and Azide free (ab247588)
    Immunoprecipitation - Anti-PAR4 antibody [EPR3991] - BSA and Azide free (ab247588)

    This data was developed using ab92590, the same antibody clone in a different buffer formulation.

    ab92590 at 1/40 immunoprecipitating PAR4 in Jurkat (human T cell leukemia T lymphocyte) whole cell lysate.

      Lane 1 (+): ab92590 + Jurkat whole cell lysate

      Lane 2 (-): PBS instead of ab92590 in Jurkat whole cell lysate

    For western blotting, ab92590 was at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (HRP), specific to the non-reduced form of IgG as the secondary antibody (1/1500).

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-PAR4 antibody [EPR3991] - BSA and Azide free (ab247588)
    Anti-PAR4 antibody [EPR3991] - BSA and Azide free (ab247588)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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