Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free (ab264485)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [C-11] to pan Cytokeratin - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free
    See all pan Cytokeratin primary antibodies

  • Description

    Mouse monoclonal [C-11] to pan Cytokeratin - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
    Unsuitable for: IHC-Fr

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • IHC-P: Human bladder carcinoma and liver tissue. Mouse and rat stomach tissues. ICC/IF: HeLa and A431 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab264485 is the carrier-free version of ab7753. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free (ab264485)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free (ab264485)

    Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue labeling pan Cytokeratin with ab7753 at 1/2000 dilution (0.28 μg/ml) followed by a ready to use secondary antibody. Membranous staining on human bladder carcinoma is observed. The section was incubated with ab7753 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use secondary.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab7753).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free (ab264485)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free (ab264485)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling pan Cytokeratin with ab7753 at 1/2000 dilution (0.28 μg/ml) followed by a ready to use secondary antibody. Membranous staining on human liver tissue is observed. The section was incubated with ab7753 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use secondary.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab7753).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free (ab264485)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free (ab264485)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling pan Cytokeratin with ab7753 at 1/2000 dilution (0.28 μg/ml) followed by a ready to use secondary. Membranous staining on mouse stomach is observed. The section was incubated with ab7753 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use secondary.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab7753).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free (ab264485)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free (ab264485)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling pan Cytokeratin with ab7753 at 1/2000 dilution (0.28 μg/ml) followed by a ready to use secondary. Membranous staining on rat stomach is observed. The section was incubated with ab7753 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab7753).

  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free (ab264485)
    Immunocytochemistry/ Immunofluorescence - Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free (ab264485)

    Immunofluorescent analysis of 100% methanol-fixed HeLa (human cervix adenocarcinoma epithelial cell) cells labeling pan Cytokeratin with ab7753 at 1/50 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HeLa cell line is observed. ab179513 Anti-beta Tubulin antibody - Microtubule Marker was used to counterstain tubulin at 1/200 dilution (Red). ab150080 AlexaFluor®594 Goat anti-Rabbit secondary was used as the secondary for the counterstain at 1/1000 dilution. The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

    -ve control 1: ab150080 at 1/1000 dilution.

    -ve control 2: ab179513 at 1/200 dilution followed by ab150113 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab7753).

  • Immunocytochemistry/ Immunofluorescence - Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free (ab264485)
    Immunocytochemistry/ Immunofluorescence - Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free (ab264485)

    Immunofluorescent analysis of 100% Methanol-fixed A431 (human epidermoid carcinoma epithelial cell) cells labeling pan Cytokeratin with ab7753 at 1/50 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A431 cell line is observed. ab179513 Anti-beta Tubulin antibody - Microtubule Marker was used to counterstain tubulin at 1/200 dilution (Red). ab150080 AlexaFluor®594 Goat anti-Rabbit secondary was used as the secondary for the counterstain at 1/1000 dilution. The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150113 AlexaFluor®488 Goat anti-Mouse secondary at 1/1000 dilution.

    -ve control 1: ab150080 at 1/1000 dilution.

    -ve control 2: ab179513 at 1/200 dilution followed by ab150113 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab7753).

  • Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free (ab264485)
    Anti-pan Cytokeratin antibody [C-11] - BSA and Azide free (ab264485)

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