Anti-PAI1 antibody [1D5] (ab125687)
![Anti-PAI1 antibody [1D5] (ab125687)](https://www.abcam.com/ps/products/125/ab125687/Images/ab125687-139710-anti-pai1-antibody-1d5-western-blot.jpg "Anti-PAI1 antibody [1D5] (ab125687)")
Key features and details
- Mouse monoclonal [1D5] to PAI1
- Suitable for: WB, ELISA, Flow Cyt, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-PAI1 antibody [1D5]
See all PAI1 primary antibodies -
Description
Mouse monoclonal [1D5] to PAI1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt MouseIHC-P HumanWB Human
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Immunogen
Purified recombinant fragment of Human PAI1 expressed in E. Coli.
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Positive control
- Recombinant Human PAI1 protein (aa194-316); PAI1 (aa194-316)-hIgGFc transfected HEK293 cell lysate; Human lung cancer and kidney cancer tissues; NIH 3T3 cells.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR201079-27 are from Tissue Culture Supernatant
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1D5 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-PAI1 antibody [1D5] (ab125687)Anti-PAI1 antibody [1D5] (ab125687) at 1/500 dilution + Human PAI1 recombinant protein (amino acids 194-316)
Predicted band size: 45 kDa
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![Western blot - Anti-PAI1 antibody [1D5] (ab125687)](https://www.abcam.com/ps/products/125/ab125687/Images/ab125687-139709-anti-pai1-antibody-1d5-western-blot.jpg)
Western blot - Anti-PAI1 antibody [1D5] (ab125687)All lanes : Anti-PAI1 antibody [1D5] (ab125687) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : PAI1-hIgGFc transfected HEK293 cell lysate (aa194-316)
Predicted band size: 45 kDa
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![ELISA - Anti-PAI1 antibody [1D5] (ab125687)](https://www.abcam.com/ps/products/125/ab125687/Images/ab125687-139708-anti-pai1-antibody-1d5-elisa.jpg)
ELISA - Anti-PAI1 antibody [1D5] (ab125687)ELISA using ab125687. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (ab125687)](https://www.abcam.com/ps/products/125/ab125687/Images/ab125687-139707-anti-pai1-antibody-1d5-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (ab125687)ab123687 at 1/200 dilution staining PAI1 in Paraffin-embedded Human lung cancer tissue by Immunohistochemistry followed by DAB staining. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (ab125687)](https://www.abcam.com/ps/products/125/ab125687/Images/ab125687-139706-anti-pai1-antibody-1d5-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAI1 antibody [1D5] (ab125687)ab123687 at 1/200 dilution staining PAI1 in Paraffin-embedded Human kidney cancer tissue by Immunohistochemistry followed by DAB staining. -
![Flow Cytometry - Anti-PAI1 antibody [1D5] (ab125687)](https://www.abcam.com/ps/products/125/ab125687/Images/ab125687-139705-anti-pai1-antibody-1d5-flow-cytometry.jpg)
Flow Cytometry - Anti-PAI1 antibody [1D5] (ab125687)ab125687 at 1/200 dilution staining PAI1 in NIH 3T3 cells by Flow Cytometry (green), negative control (red).
