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Signal Transduction Protein Phosphorylation Ser / Thr Kinases S6K Family

Anti-P70 S6 Kinase beta/SRK antibody (ab70271)

Anti-P70 S6 Kinase beta/SRK antibody (ab70271)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to P70 S6 Kinase beta/SRK
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-P70 S6 Kinase beta/SRK antibody
    See all P70 S6 Kinase beta/SRK primary antibodies
  • Description

    Rabbit polyclonal to P70 S6 Kinase beta/SRK
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human P70 S6 Kinase beta/SRK aa 430-482 (C terminal). The exact sequence is proprietary.
    Database link: NP_003943.2

  • Positive control

    • HeLa whole cell lysate
  • General notes

    Previously labelled as P70 S6 Kinase beta. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • S6K Family
    • Metabolism
    • Types of disease
    • Diabetes
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Metabolic disorders

Images

  • Immunoprecipitation - Anti-P70 S6 Kinase beta/SRK antibody (ab70271)
    Immunoprecipitation - Anti-P70 S6 Kinase beta/SRK antibody (ab70271)
    Detection of P70 S6 Kinase beta/SRK by western blot of immunoprecipitates.
    Samples: Lane 1: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa (human epithelial cell line from cervix adenocarcinoma) cells prepared using NETN lysis buffer. Lane 2: Control IgG.
    Antibodies: ab70271 used for IP at 3 µg per reaction. For blotting immunoprecipitated P70 S6 Kinase beta/SRK, ab70271 was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
  • Western blot - Anti-P70 S6 Kinase beta/SRK antibody (ab70271)
    Western blot - Anti-P70 S6 Kinase beta/SRK antibody (ab70271)
    All lanes : Anti-P70 S6 Kinase beta/SRK antibody (ab70271) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 15 µg
    Lane 2 : HeLa whole cell lysate at 5 µg

    Predicted band size: 53 kDa
    Observed band size: 53 kDa
    Additional bands at: 35 kDa, 67 kDa, 69 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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