Anti-P70 S6 Kinase beta/SRK antibody (ab70271)
Key features and details
- Rabbit polyclonal to P70 S6 Kinase beta/SRK
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-P70 S6 Kinase beta/SRK antibody
See all P70 S6 Kinase beta/SRK primary antibodies -
Description
Rabbit polyclonal to P70 S6 Kinase beta/SRK -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Synthetic peptide within Human P70 S6 Kinase beta/SRK aa 430-482 (C terminal). The exact sequence is proprietary.
Database link: NP_003943.2 -
Positive control
- HeLa whole cell lysate
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General notes
Previously labelled as P70 S6 Kinase beta.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Detection of P70 S6 Kinase beta/SRK by western blot of immunoprecipitates.
Samples: Lane 1: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa (human epithelial cell line from cervix adenocarcinoma) cells prepared using NETN lysis buffer. Lane 2: Control IgG.
Antibodies: ab70271 used for IP at 3 µg per reaction. For blotting immunoprecipitated P70 S6 Kinase beta/SRK, ab70271 was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds. -
All lanes : Anti-P70 S6 Kinase beta/SRK antibody (ab70271) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 15 µg
Lane 2 : HeLa whole cell lysate at 5 µg
Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 35 kDa, 67 kDa, 69 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds