Anti-p60 CAF1/MPP7 antibody (ab72520)
Key features and details
- Rabbit polyclonal to p60 CAF1/MPP7
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-p60 CAF1/MPP7 antibody
See all p60 CAF1/MPP7 primary antibodies -
Description
Rabbit polyclonal to p60 CAF1/MPP7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rhesus monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide corresponding to Human p60 CAF1/MPP7 (C terminal).
Database link: NP_005432.1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p60 CAF1/MPP7 antibody (ab72520)All lanes : Anti-p60 CAF1/MPP7 antibody (ab72520) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 240 kDa, 25 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody (ab72520)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer tissue labelling p60 CAF1/MPP7 with ab7252 at 1/200 (1µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-p60 CAF1/MPP7 antibody (ab72520)Immunoprecipitation/ Western Blot of p60 CAF1/MPP7. Lane 1: ab72520 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab72520 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 3 seconds.
