Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸ 10 µl 127 315 ₸

Order now and get it on Tuesday March 02, 2021

Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6105] to p60 CAF1/MPP7
Suitable for: WB, IP, IHC-P, Flow Cyt
Reacts with: Human

Product name

Anti-p60 CAF1/MPP7 antibody [EPR6105]
See all p60 CAF1/MPP7 primary antibodies
Description

Rabbit monoclonal [EPR6105] to p60 CAF1/MPP7
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Jurkat, HeLa, 293T, and Caco2 cell lysates; Human colon and tonsil tissues.
General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

This product was previously labelled as p60 CAF1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR6105
Isotype

IgG
Research areas

Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

All lanes : Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Caco2 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 61 kDa
Observed band size: 70 kDa
why is the actual band size different from the predicted?
Flow Cytometry - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

Overlay histogram showing HeLa cells stained with ab109442 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109442, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

ab109442 at 1/250 dilution staining p60 CAF1/MPP7 in paraffin-embedded Human colon tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

ab109442 at 1/250 dilution staining p60 CAF1/MPP7 in paraffin-embedded Human tonsil tissue.

Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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