Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6105] to p60 CAF1/MPP7
  • Suitable for: WB, IP, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-p60 CAF1/MPP7 antibody [EPR6105]
    See all p60 CAF1/MPP7 primary antibodies

  • Description

    Rabbit monoclonal [EPR6105] to p60 CAF1/MPP7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, HeLa, 293T, and Caco2 cell lysates; Human colon and tonsil tissues.

  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

     This product was previously labelled as p60 CAF1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR6105

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
    Western blot - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
    All lanes : Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 293T cell lysate
    Lane 4 : Caco2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 61 kDa
    Observed band size: 70 kDa
    why is the actual band size different from the predicted?

  • Flow Cytometry - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
    Flow Cytometry - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
    Overlay histogram showing HeLa cells stained with ab109442 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109442, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

    ab109442 at 1/250 dilution staining p60 CAF1/MPP7 in paraffin-embedded Human colon tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

    ab109442 at 1/250 dilution staining p60 CAF1/MPP7 in paraffin-embedded Human tonsil tissue.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)
    Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-p60 CAF1/MPP7 antibody [EPR6105] (ab109442)

© 2021 Astana Biomed Group LLP. All rights reserved.