cGMP Assay Kit - Direct Immunoassay (ab65356)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr
- Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.008 µM
Overview
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Product name
cGMP Assay Kit - Direct Immunoassay -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Quantitative -
Sensitivity
> 0.008 µM -
Range
0.008 µM - 2 µM -
Assay time
3h 00m -
Product overview
cGMP Assay Kit ab65356 provides a direct competitive immunoassay for sensitive and quantitative determination of cGMP level in biological samples.
The cGMP assay protocol uses the recombinant Protein G coated plate to anchor cGMP polyclonal antibody. cGMP-HRP conjugates directly competes with cGMP from samples for binding to the cGMP specific antibody on the plate. After incubation and washing, the amount of cGMP-HRP bound to plate can easily be determined by reading OD450nm. The intensity of OD450nm is inversely proportional to the concentration of cGMP in samples.
The kit provides a new acetylation procedure that improves detection signal significantly. The kit can detect 0.04-10 pmol/well (0.008-2 µM) cGMP samples.
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Notes
Previously called cGMP Direct Immunoassay Kit.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Storage buffer
Preservative: None
Constituents: BSA -
Components Identifier 100 tests 10X cGMP Assay Buffer WM 1 x 25ml Acetylating Reagent A Violet 1 x 750µl Acetylating Reagent B Black 1 x 1.5ml Anti-cGMP pAb/BSA Red 1 vial cGMP-HRP/BSA Green 1 vial HRP Developer Amber 1 x 10ml Neutralizing Buffer NM 1 x 7.5ml Protein G Coated Plate 1 pack Standard cGMP (10 nmol) Yellow 1 vial -
Research areas
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Relevance
Cyclic guanosine monophosphate (cGMP) is a cyclic nucleotide derived from guanosine triphosphate (GTP). It serves as a second messenger in a manner similar to that observed with cAMP. Peptide hormones, such as the natriuretic factors, activate receptors that are associated with membrane-bound guanylate cyclase (GC). Receptor activation of GC leads to the conversion of GTP to cGMP. Nitric oxide (NO) also stimulates cGMP production by activating soluble GC, perhaps by binding to the heme moiety of the enzyme. Similar to cAMP, cGMP mediates most of its intracellular effects through the activation of specific cGMP dependent protein kinases (PKG). -
Alternative names
- Cyclic GMP
- Cyclic guanosine monophosphate
- Guanosine 3’ 5’ cyclic monophosphate
Images
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Functional studies - ab65356 cGMP Direct Immunoassay Kit
O.D.'s of human urine measured at various dilutions (+/- SD)
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Functional studies - ab65356 cGMP Direct Immunoassay KitStandard curve showing mean of duplicates (+/-SD) with background readings subtracted
