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Anti-p27 KIP 1 (phospho T198) antibody (ab64949)

Price and availability

284 784 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-p27 KIP 1 (phospho T198) antibody (ab64949)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to p27 KIP 1 (phospho T198)
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-p27 KIP 1 (phospho T198) antibody
    See all p27 KIP 1 primary antibodies
  • Description

    Rabbit polyclonal to p27 KIP 1 (phospho T198)
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of p27 Kip 1 only when phosphorylated at threonine 198.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic phosphopeptide (Human) from around the phosphorylation site of threonine 198 (RRRQTP)

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Cip / Kip
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • Cip/Kip
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Cip/kip

Images

  • Western blot - Anti-p27 KIP 1 (phospho T198) antibody (ab64949)
    Western blot - Anti-p27 KIP 1 (phospho T198) antibody (ab64949)
    All lanes : Anti-p27 KIP 1 (phospho T198) antibody (ab64949) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HepG2 cell extract
    Lane 3 : COLO205 cell extract
    Lane 4 : COLO205 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 22 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-p27 KIP 1 (phospho T198) antibody (ab64949)
    Western blot - Anti-p27 KIP 1 (phospho T198) antibody (ab64949) This image is courtesy of an anonymous Abreview
    Anti-p27 KIP 1 (phospho T198) antibody (ab64949) at 1/1000 dilution + Mouse liver tissue lysate at 40 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 34 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second

    See Abreview

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-p27 KIP 1 (phospho T198) antibody (ab64949)

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    Applications: ICC/IF, IHC-P, IP, WB

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    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

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    Applications: IHC-P, WB

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