Anti-p27 KIP 1 antibody [57/Kip1/p27] (ab280580)
![Anti-p27 KIP 1 antibody [57/Kip1/p27] (ab280580)](https://www.abcam.com/ps/products/280/ab280580/Images/ab280580-459725-anti-p27-kip-1-antibody-57kip1p27-western-blot-hela1.jpg "Anti-p27 KIP 1 antibody [57/Kip1/p27] (ab280580)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [57/Kip1/p27] to p27 KIP 1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-p27 KIP 1 antibody [57/Kip1/p27]
See all p27 KIP 1 primary antibodies -
Description
Mouse monoclonal [57/Kip1/p27] to p27 KIP 1 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- WB: HeLa, Jurkat, Raji, NIH/3T3 and PC-12 whole cell lysates.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
57/Kip1/p27 -
Isotype
IgG1
Images
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Western blot - Anti-p27 KIP 1 antibody [57/Kip1/p27] (ab280580)All lanes : Anti-p27 KIP 1 antibody [57/Kip1/p27] (ab280580) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 3 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate
Lane 4 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Daudi cells should have very little/negative expression.
Exposure time: 3 minutes.
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![Western blot - Anti-p27 KIP 1 antibody [57/Kip1/p27] (ab280580)](https://www.abcam.com/ps/products/280/ab280580/Images/ab280580-459726-anti-p27-kip-1-antibody-57kip1p27-western-blot-nih3t31.jpg)
Western blot - Anti-p27 KIP 1 antibody [57/Kip1/p27] (ab280580)All lanes : Anti-p27 KIP 1 antibody [57/Kip1/p27] (ab280580) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 2 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Anti-p27 KIP 1 antibody [57/Kip1/p27] (ab280580)](https://www.abcam.com/ps/products/280/ab280580/Images/ab280580-459738-Benefits-of-Recombinant-antibodies.jpg)
Anti-p27 KIP 1 antibody [57/Kip1/p27] (ab280580)