Anti-Obestatin antibody [EPR19972] (ab216575)
![Anti-Obestatin antibody [EPR19972] (ab216575)](https://www.abcam.com/ps/products/216/ab216575/Images/ab216575-270826-anti-obestatin-antibody-epr19972-western-blot.jpg "Anti-Obestatin antibody [EPR19972] (ab216575)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19972] to Obestatin
- Suitable for: WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Obestatin antibody [EPR19972]
See all Obestatin primary antibodies -
Description
Rabbit monoclonal [EPR19972] to Obestatin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseIHC-P MouseHumanWB Human
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Positive control
- WB: GST and His-tagged human Appetite-regulating hormone, full length recombinant protein (aa1-117); His-tagged human Obestatin peptide (aa76-98)*5. IHC-P: Human stomach and duodenum tissues; Mouse stomach tissue. IHC-Fr: Mouse stomach tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19972 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Obestatin antibody [EPR19972] (ab216575)All lanes : Anti-Obestatin antibody [EPR19972] (ab216575) at 1/1000 dilution
Lane 1 : GST and His-tagged human Appetite-regulating hormone, full length recombinant protein (aa1-117) at 0.005 µg
Lane 2 : His-tagged human Obestatin peptide (aa76-98)*5 at 0.005 µg
Lane 3 : His-tagged human Ghrelin peptide (aa24-51)*4 at 0.05 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 13 kDa
Observed band size: 13,38 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
Obestatin and Ghrelin are processed cleaved forms deriving from Appetite-regulating hormone. This product is specific to Obestatin.
Detecting Obestatin in endogenous tissues may require optimization.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] (ab216575)](https://www.abcam.com/ps/products/216/ab216575/Images/ab216575-270825-anti-obestatin-antibody-epr19972-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] (ab216575)Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Obestatin with ab216575 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.
Cytoplasmic staining on endocrine cells of human stomach is observed [PMID: 18474938].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] (ab216575)](https://www.abcam.com/ps/products/216/ab216575/Images/ab216575-270823-anti-obestatin-antibody-epr19972-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] (ab216575)Immunohistochemical analysis of paraffin-embedded human duodenum tissue labeling Obestatin with ab216575 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.
Cytoplasmic staining on endocrine cells of human duodenum is observed [PMID: 18474938].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] (ab216575)](https://www.abcam.com/ps/products/216/ab216575/Images/ab216575-270820-anti-obestatin-antibody-epr19972-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] (ab216575)Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Obestatin with ab216575 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.
Cytoplasmic staining on endocrine cells of mouse stomach is observed [PMID: 18474938].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] (ab216575)](https://www.abcam.com/ps/products/216/ab216575/Images/ab216575-270819-anti-obestatin-antibody-epr19972-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] (ab216575)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Obestatin with ab216575 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.
Negative control: no staining on human colon [PMID: 18474938].
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Frozen sections) - Anti-Obestatin antibody [EPR19972] (ab216575)](https://www.abcam.com/ps/products/216/ab216575/Images/ab216575-270818-anti-obestatin-antibody-epr19972-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Obestatin antibody [EPR19972] (ab216575)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling Obestatin with ab216575 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Cytoplasmic staining on endocrine cells of mouse stomach is observed PMID: 18474938].
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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![Anti-Obestatin antibody [EPR19972] (ab216575)](https://www.abcam.com/ps/products/216/ab216575/Images/ab216575-7-benefits-of-recombinant-antibodies.png)
Anti-Obestatin antibody [EPR19972] (ab216575)