Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Obestatin antibody [EPR19972] (ab216575)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19972] to Obestatin
Suitable for: WB, IHC-P, IHC-Fr
Reacts with: Mouse, Human

Product name

Anti-Obestatin antibody [EPR19972]
See all Obestatin primary antibodies
Description

Rabbit monoclonal [EPR19972] to Obestatin
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse
IHC-P	Mouse Human
WB	Human

See all applications and species data

Positive control
- WB: GST and His-tagged human Appetite-regulating hormone, full length recombinant protein (aa1-117); His-tagged human Obestatin peptide (aa76-98)*5. IHC-P: Human stomach and duodenum tissues; Mouse stomach tissue. IHC-Fr: Mouse stomach tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19972
Isotype

IgG
Research areas

Western blot - Anti-Obestatin antibody [EPR19972] (ab216575)

All lanes : Anti-Obestatin antibody [EPR19972] (ab216575) at 1/1000 dilution

Lane 1 : GST and His-tagged human Appetite-regulating hormone, full length recombinant protein (aa1-117) at 0.005 µg
Lane 2 : His-tagged human Obestatin peptide (aa76-98)*5 at 0.005 µg
Lane 3 : His-tagged human Ghrelin peptide (aa24-51)*4 at 0.05 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 13 kDa
Observed band size: 13,38 kDa
why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.

Obestatin and Ghrelin are processed cleaved forms deriving from Appetite-regulating hormone. This product is specific to Obestatin.

Detecting Obestatin in endogenous tissues may require optimization.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] (ab216575)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Obestatin with ab216575 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.

Cytoplasmic staining on endocrine cells of human stomach is observed [PMID: 18474938].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] (ab216575)

Immunohistochemical analysis of paraffin-embedded human duodenum tissue labeling Obestatin with ab216575 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.

Cytoplasmic staining on endocrine cells of human duodenum is observed [PMID: 18474938].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] (ab216575)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Obestatin with ab216575 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.

Cytoplasmic staining on endocrine cells of mouse stomach is observed [PMID: 18474938].

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Obestatin antibody [EPR19972] (ab216575)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Obestatin with ab216575 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at a ready to use dilution.

Negative control: no staining on human colon [PMID: 18474938].

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Obestatin antibody [EPR19972] (ab216575)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling Obestatin with ab216575 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Cytoplasmic staining on endocrine cells of mouse stomach is observed PMID: 18474938].

The nuclear counterstain is DAPI (blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Anti-Obestatin antibody [EPR19972] (ab216575)

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