Anti-NT5C3 antibody [009] - BSA and Azide free (ab276067)
![Anti-NT5C3 antibody [009] - BSA and Azide free (ab276067)](https://www.abcam.com/ps/products/276/ab276067/Images/ab276067-2-anti-nt5c3a-antibody-ip-hek293t-jurkat-cells.jpg "Anti-NT5C3 antibody [009] - BSA and Azide free (ab276067)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [009] to NT5C3 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-NT5C3 antibody [009] - BSA and Azide free -
Description
Rabbit monoclonal [009] to NT5C3 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human NT5C3 aa 12-297.
Database link: Q9H0P0 -
Positive control
- WB: HepG2 whole cell lysate. IP: HEK-293T and Jurkat whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
009 -
Isotype
IgG
Images
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Immunoprecipitation - Anti-NT5C3 antibody [009] (ab276067)
NT5C3 was immunoprecipitated from 0.5 mg HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) and Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with 0.5 µL ab276067 and 15 μl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using ab276067 at 1/500 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.
Developed using the odssey technique.
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![Western blot - Anti-NT5C3 antibody [009] (ab276067)](https://www.abcam.com/ps/products/276/ab276067/Images/ab276067-1-anti-nt5c3a-antibody-wb-hepg2-cells.jpg)
Western blot - Anti-NT5C3 antibody [009] (ab276067)Anti-NT5C3 antibody [009] - BSA and Azide free (ab276067) at 1/500 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Secondary
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution
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![Anti-NT5C3 antibody [009] - BSA and Azide free (ab276067)](https://www.abcam.com/ps/products/276/ab276067/Images/ab276067-3-benefits-of-recombinant-antibodies.png)
Anti-NT5C3 antibody [009] - BSA and Azide free (ab276067)