Anti-NPHS2 antibody (ab177242)
Key features and details
- Goat polyclonal to NPHS2
- Suitable for: WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-NPHS2 antibody
See all NPHS2 primary antibodies -
Description
Goat polyclonal to NPHS2 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHumanPig
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Immunogen
Synthetic peptide corresponding to Human NPHS2 aa 365-377 (C terminal) (Cysteine residue).
Sequence:SPSKPVEPLNPKK
Database link: NP_055440.1 -
Positive control
- WB: Mouse, human and pig kidney lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab177242 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NPHS2 antibody (ab177242)All lanes : Anti-NPHS2 antibody (ab177242) at 0.3 µg/ml
Lane 1 : Human kidney lysate (in RIPA buffer)
Lane 2 : Mouse kidney lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
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Western blot - Anti-NPHS2 antibody (ab177242)Anti-NPHS2 antibody (ab177242) at 1 µg/ml + Pig kidney lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 42 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
