Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Nogo Receptor antibody [EPR11159] (ab184556)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11159] to Nogo Receptor
Suitable for: WB, IP
Reacts with: Human

Product name

Anti-Nogo Receptor antibody [EPR11159]
See all Nogo Receptor primary antibodies
Description

Rabbit monoclonal [EPR11159] to Nogo Receptor
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- SH-SY5Y, 293 and HepG2 cell lysates. Neuro-2a and C6 whole cell lysates. Mouse brain and hippocampus tissue lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR11159
Isotype

IgG
Research areas

Western blot - Anti-Nogo Receptor antibody [EPR11159] (ab184556)

All lanes : Anti-Nogo Receptor antibody [EPR11159] (ab184556) at 1/5000 dilution

Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse hippocampus tissue lysate
Lane 4 : C6 (rat glial tumor glial cell) whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 51 kDa
Western blot - Anti-Nogo Receptor antibody [EPR11159] (ab184556)

All lanes : Anti-Nogo Receptor antibody [EPR11159] (ab184556) at 1/50000 dilution

Lane 1 : SH-SY5Y cell lysate
Lane 2 : HepG2 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 51 kDa
Observed band size: 66 kDa
why is the actual band size different from the predicted?
Western blot - Anti-Nogo Receptor antibody [EPR11159] (ab184556)

Anti-Nogo Receptor antibody [EPR11159] (ab184556) at 1/10000 dilution + 293 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 51 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?
Immunoprecipitation - Anti-Nogo Receptor antibody [EPR11159] (ab184556)

Western blot analysis of Nogo Receptor in HepG2 cell lysate immunoprecipitated with ab184556 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
Anti-Nogo Receptor antibody [EPR11159] (ab184556)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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