Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Nogo antibody [EPR12265] - BSA and Azide free (ab250250)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12265] to Nogo - BSA and Azide free
  • Suitable for: ICC, IP, Flow Cyt, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Nogo antibody [EPR12265] - BSA and Azide free
    See all Nogo primary antibodies

  • Description

    Rabbit monoclonal [EPR12265] to Nogo - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, Flow Cyt, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250250 is the carrier-free version of ab180847 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250250 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Nogo antibody [EPR12265] - BSA and Azide free (ab250250)
    Western blot - Anti-Nogo antibody [EPR12265] - BSA and Azide free (ab250250)
    All lanes : Anti-Nogo antibody [EPR12265] (ab180847) at 1/1000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : SH-SY5Y cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 130 kDa
    Observed band size: 40-55 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab180847, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nogo antibody [EPR12265] - BSA and Azide free (ab250250)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nogo antibody [EPR12265] - BSA and Azide free (ab250250)
    This data was developed using ab180847, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human brain tissue labeling Nogo with ab180847 at a 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nogo antibody [EPR12265] - BSA and Azide free (ab250250)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nogo antibody [EPR12265] - BSA and Azide free (ab250250)
    This data was developed using ab180847, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded, Human breast carcinoma tissue labeling Nogo with ab180847 at a 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Nogo antibody [EPR12265] - BSA and Azide free (ab250250)
    Immunocytochemistry - Anti-Nogo antibody [EPR12265] - BSA and Azide free (ab250250)
    This data was developed using ab180847, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of U87-MG cells labeling Nogo with ab180847 at a 1/100 dilution.
  • Flow Cytometry - Anti-Nogo antibody [EPR12265] - BSA and Azide free (ab250250)
    Flow Cytometry - Anti-Nogo antibody [EPR12265] - BSA and Azide free (ab250250)
    This data was developed using ab180847, the same antibody clone in a different buffer formulation.Flow cytometry analysis of permeabilized HeLa cells labeling Nogo (red) with ab180847 at a 1/10 dilution, or negative control rabbit IgG (green)
  • Immunoprecipitation - Anti-Nogo antibody [EPR12265] - BSA and Azide free (ab250250)
    Immunoprecipitation - Anti-Nogo antibody [EPR12265] - BSA and Azide free (ab250250)

    This data was developed using ab180847, the same antibody clone in a different buffer formulation.

    Western blot analysis on Immunoprecipitation pellet from either 1) HeLa cell lysate, or 2) 1xPBS (negative control); showing Nogo, using ab180847 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-Nogo antibody [EPR12265] - BSA and Azide free (ab250250)
    Anti-Nogo antibody [EPR12265] - BSA and Azide free (ab250250)

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