Anti-Nodal antibody (ab227052)
Key features and details
- Rabbit polyclonal to Nodal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Nodal antibody
See all Nodal primary antibodies -
Description
Rabbit polyclonal to Nodal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Recombinant fragment within Human Nodal (internal sequence). The exact sequence is proprietary.
Database link: Q96S42 -
Positive control
- IHC-P: Human breast cancer tissue; rat brain tissue. WB: Human ESC whole cell lysate; mouse brain tissue extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Anti-Nodal antibody (ab227052) at 1/1000 dilution + Human embryonic stem cell (ESC) whole cell lysate at 1/20 dilution
Developed using the ECL technique.
Predicted band size: 40 kDa10% SDS PAGE
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Paraffin embedded human breast cancer tissue stained for Nodal with ab227052 (1/250 dilution) in immunohistochemical analysis.
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Paraffin embedded rat brain tissue stained for Nodal with ab227052 (1/500 dilution) in immunohistochemical analysis.
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Anti-Nodal antibody (ab227052) at 1/500 dilution + Mouse brain tissue extracts at 50 µg
Developed using the ECL technique.
Predicted band size: 40 kDa10% SDS-PAGE