Anti-Atrophin 1 antibody (ab32604)
Key features and details
- Rabbit polyclonal to Atrophin 1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Atrophin 1 antibody
See all Atrophin 1 primary antibodies -
Description
Rabbit polyclonal to Atrophin 1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1150 to the C-terminus of Human Atrophin 1.
Read Abcam's proprietary immunogen policy (Peptide available as ab32861.) -
Positive control
- This antibody gave a positive signal in Human Testis Tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab32604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. WB Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 125 kDa). Target
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Relevance
Atrophin 1 (ATN1) is essential for the development of multiple tissues. Defects in ATN1 are the cause of dentatorubral-pallidoluysian atrophy (DRPLA). -
Cellular localization
Cytoplasm. Nuclear -
Database links
- Entrez Gene: 1822 Human
- Entrez Gene: 13498 Mouse
- Entrez Gene: 29515 Rat
- Omim: 607462 Human
- SwissProt: P54259 Human
- SwissProt: O35126 Mouse
- SwissProt: P54258 Rat
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Alternative names
- ATN1 antibody
- Atrophin1 antibody
- B37 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Atrophin 1 antibody (ab32604)IHC image of ab32604 staining in Human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32604, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-Atrophin 1 antibody (ab32604)Anti-Atrophin 1 antibody (ab32604) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 125 kDa
Observed band size: 125 kDa
Additional bands at: 100 kDa, 55 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
References (0)
ab32604 has not yet been referenced specifically in any publications.
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Atrophin 1 antibody (ab32604)IHC image of ab32604 staining in Human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32604, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-Atrophin 1 antibody (ab32604)Anti-Atrophin 1 antibody (ab32604) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Predicted band size: 125 kDa
Observed band size: 125 kDa
Additional bands at: 100 kDa, 55 kDa, 76 kDa. We are unsure as to the identity of these extra bands.
