Anti-Lactate Dehydrogenase antibody (ab231903)
Key features and details
- Rabbit polyclonal to Lactate Dehydrogenase
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-Lactate Dehydrogenase antibody
See all Lactate Dehydrogenase primary antibodies -
Description
Rabbit polyclonal to Lactate Dehydrogenase -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human, Pig
Predicted to work with: Rat, Rabbit, Cow, Chimpanzee, Macaque monkey, Orangutan
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Immunogen
Recombinant full length protein corresponding to Pig Lactate Dehydrogenase aa 1 to the C-terminus. N-terminal His-tag and GST-tag. Expressed in E. coli.
Database link: P00339 -
Positive control
- WB: Recombinant porcine Lactate Dehydrogenase protein; porcine serum and kidney tissue lysate; mouse testis tissue lysate; A431 and A375 whole cell lysate. IHC-P: Porcine skeletal muscle and heart tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody (ab231903)
Formalin-fixed, paraffin-embedded porcine skeletal muscle tissue stained for Lactate Dehydrogenase using ab231903 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-Lactate Dehydrogenase antibody (ab231903)All lanes : Anti-Lactate Dehydrogenase antibody (ab231903) at 3 µg/ml
Lane 1 : Porcine serum
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : A-375 (human malignant melanoma cell line) whole cell lysate
Lane 4 : Porcine kidney tissue lysate
Lane 5 : Mouse testis tissue lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lactate Dehydrogenase antibody (ab231903)Formalin-fixed, paraffin-embedded porcine heart tissue stained for Lactate Dehydrogenase using ab231903 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-Lactate Dehydrogenase antibody (ab231903)Anti-Lactate Dehydrogenase antibody (ab231903) at 3 µg/ml + Recombinant porcine Lactate Dehydrogenase protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/1000 dilution
Predicted band size: 37 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
