Anti-NNMT antibody [OTI3D8] (ab119758)
![Anti-NNMT antibody [OTI3D8] (ab119758)](https://www.abcam.com/ps/products/119/ab119758/Images/ab119758-395421-anti-nnmt-antibody-oti3d8-western-blot-human-knockout-lysate.jpg "Anti-NNMT antibody [OTI3D8] (ab119758)")
Key features and details
- Mouse monoclonal [OTI3D8] to NNMT
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Knockout validated
- Reacts with: Mouse, Rat, Dog, Human, African green monkey
- Isotype: IgG2b
Overview
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Product name
Anti-NNMT antibody [OTI3D8]
See all NNMT primary antibodies -
Description
Mouse monoclonal [OTI3D8] to NNMT -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF African green monkeyIHC-P HumanWB Human
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Immunogen
Recombinant full length Human NNMT, produced in HEK293T cells (NP_006160).
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Positive control
- WB: HeLa, A549, HepG2 and SVT2 cell lysates; HEK293T cell lysate transfected with pCMV6-ENTRY NNMT cDNA. IHC-P: Human Liver, Pancreas, Thyroid carcinoma, Endometrium, Endometrium adenocarcinoma and Prostate tissues. ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY NNMT. Flow Cyt: Jurkat cells.
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General notes
The clone number has been updated from 3D8 to OTI3D8, both clone numbers name the same clone.
This product was changed from ascites to tissue culture supernatant on 29th May 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab119758 is purified from TCS by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI3D8 -
Isotype
IgG2b -
Research areas
Images
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Western blot - Anti-NNMT antibody [OTI3D8] (ab119758)All lanes : Anti-NNMT antibody [OTI3D8] (ab119758) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : NNMT knockout HeLa cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDaLanes 1-3: Merged signal (red and green). Green - ab119758 observed at 30 kDa. Red - loading control ab181602 observed at 36 kDa.
ab119758 Anti-NNMT antibody [OTI3D8] was shown to specifically react with NNMT in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265700 (knockout cell lysate ab258537) was used. Wild-type and NNMT knockout samples were subjected to SDS-PAGE. ab119758 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated at room temperature for 2.5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNMT antibody [OTI3D8] (ab119758)](https://www.abcam.com/ps/products/119/ab119758/Images/ab119758-132938-anti-nnmt-antibody-oti3d8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNMT antibody [OTI3D8] (ab119758)ab119758 at 1/150 dilution, staining NNMT in paraffin-embedded Human liver tissue by Immunohistochemistry. -
![Western blot - Anti-NNMT antibody [OTI3D8] (ab119758)](https://www.abcam.com/ps/products/119/ab119758/Images/ab119758-132940-anti-nnmt-antibody-oti3d8-western-blot.jpg)
Western blot - Anti-NNMT antibody [OTI3D8] (ab119758)All lanes : Anti-NNMT antibody [OTI3D8] (ab119758) at 1/500 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY NNMT cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 30 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200641) -
![Western blot - Anti-NNMT antibody [OTI3D8] (ab119758)](https://www.abcam.com/ps/products/119/ab119758/Images/ab119758-132939-anti-nnmt-antibody-oti3d8-western-blot.jpg)
Western blot - Anti-NNMT antibody [OTI3D8] (ab119758)All lanes : Anti-NNMT antibody [OTI3D8] (ab119758) at 1/500 dilution
Lane 1 : HepG2 cell extracts
Lane 2 : HeLa cell extracts
Lane 3 : SVT2 cell extracts
Lane 4 : A549 cell extracts
Lane 5 : COS7 cell extracts
Lane 6 : Jurkat cell extracts
Lane 7 : MDCK cell extracts
Lane 8 : PC12 cell extracts
Lane 9 : MCF7 cell extracts
Lysates/proteins at 35 µg per lane.
Predicted band size: 30 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200641) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNMT antibody [OTI3D8] (ab119758)](https://www.abcam.com/ps/products/119/ab119758/Images/ab119758-132937-anti-nnmt-antibody-oti3d8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNMT antibody [OTI3D8] (ab119758)ab119758 at 1/150 dilution, staining NNMT in paraffin-embedded Human pancreas tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNMT antibody [OTI3D8] (ab119758)](https://www.abcam.com/ps/products/119/ab119758/Images/ab119758-132936-anti-nnmt-antibody-oti3d8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNMT antibody [OTI3D8] (ab119758)ab119758 at 1/150 dilution, staining NNMT in paraffin-embedded Human thyroid carcinoma tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNMT antibody [OTI3D8] (ab119758)](https://www.abcam.com/ps/products/119/ab119758/Images/ab119758-132935-anti-nnmt-antibody-oti3d8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNMT antibody [OTI3D8] (ab119758)ab119758 at 1/150 dilution, staining NNMT in paraffin-embedded Human endometrium tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNMT antibody [OTI3D8] (ab119758)](https://www.abcam.com/ps/products/119/ab119758/Images/ab119758-132934-anti-nnmt-antibody-oti3d8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNMT antibody [OTI3D8] (ab119758)ab119758 at 1/150 dilution, staining NNMT in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNMT antibody [OTI3D8] (ab119758)](https://www.abcam.com/ps/products/119/ab119758/Images/ab119758-132933-anti-nnmt-antibody-oti3d8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NNMT antibody [OTI3D8] (ab119758)ab119758 at 1/150 dilution, staining NNMT in paraffin-embedded Human prostate tissue by Immunohistochemistry. -
![Immunocytochemistry/ Immunofluorescence - Anti-NNMT antibody [OTI3D8] (ab119758)](https://www.abcam.com/ps/products/119/ab119758/Images/ab119758-132932-anti-nnmt-antibody-oti3d8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-NNMT antibody [OTI3D8] (ab119758)ab119758, at 1/100 dilution staining NNMT, in COS7 cells transiently transfected by pCMV6-ENTRY NNMT, by Immunofluorescence -
![Flow Cytometry - Anti-NNMT antibody [OTI3D8] (ab119758)](https://www.abcam.com/ps/products/119/ab119758/Images/ab119758-132931-anti-nnmt-antibody-oti3d8-flow-cytometry.jpg)
Flow Cytometry - Anti-NNMT antibody [OTI3D8] (ab119758)ab119758 at 1/100 dilution staining NNMT in Jurkat cells by Flow cytometry (Red) compared to a nonspecific negative control antibody (Blue).
