Anti-NLRC3 antibody (ab77817)
Key features and details
- Rabbit polyclonal to NLRC3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NLRC3 antibody
See all NLRC3 primary antibodies -
Description
Rabbit polyclonal to NLRC3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human NLRC3 aa 1050 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab90252) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: Raji; Ramos; Jurkat.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MousePigApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa (predicted molecular weight: 114 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 111 kDa (predicted molecular weight: 114 kDa).Target
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Function
May modulate T-cell activation. Decreases the transcription of genes that are normally up-regulated after T-cell stimulation. Delays degradation of NFKBIA/IKBA. -
Tissue specificity
Detected in peripheral blood mononuclear cells. -
Sequence similarities
Belongs to the NLRP family.
Contains 16 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 197358 Human
- Entrez Gene: 268857 Mouse
- SwissProt: Q7RTR2 Human
- SwissProt: Q5DU56 Mouse
- Unigene: 2061102 Human
- Unigene: 592091 Human
- Unigene: 741496 Human
- Unigene: 215994 Mouse
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Alternative names
- CARD15-like antibody
- CARD15-like protein antibody
- Caterpiller 16.2 antibody
see all
Images
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Western blot - Anti-NLRC3 antibody (ab77817)All lanes : ab77817 at 1 µg/ml
Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 33 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutesNLRC3 has four possible isoforms which have the following predicted molecular weights, 114, 119, 83 and 111 kDa (SwissProt data).
Datasheets and documents
References (3)
ab77817 has been referenced in 3 publications.
- Eren E et al. NLRC3 protein inhibits inflammation by disrupting NALP3 inflammasome assembly via competition with the adaptor protein ASC for pro-caspase-1 binding. J Biol Chem 292:12691-12701 (2017). PubMed: 28584053
- Guo L et al. NLRC3 promotes host resistance against Pseudomonas aeruginosa-induced keratitis by promoting the degradation of IRAK1. Int J Mol Med 40:898-906 (2017). PubMed: 28731142
- Gültekin Y et al. Overexpressed NLRC3 acts as an anti-inflammatory cytosolic protein. J Innate Immun 7:25-36 (2015). PubMed: 25277106
Images
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Western blot - Anti-NLRC3 antibody (ab77817)All lanes : ab77817 at 1 µg/ml
Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 105 kDa why is the actual band size different from the predicted?
Additional bands at: 33 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutesNLRC3 has four possible isoforms which have the following predicted molecular weights, 114, 119, 83 and 111 kDa (SwissProt data).
