Antibodies, Proteins, Kits and Reagents for Life Science

294 835 ₸ Product size

100 µl 294 835 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Nkx3.1 antibody [EPR16653] (ab196020)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16653] to Nkx3.1
Suitable for: IHC-P, WB
Reacts with: Human

Product name

Anti-Nkx3.1 antibody [EPR16653]
See all Nkx3.1 primary antibodies
Description

Rabbit monoclonal [EPR16653] to Nkx3.1
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human prostate lysate; LNCaP cell lysate; Human prostate hyperplasia tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16653
Isotype

IgG
Research areas

Western blot - Anti-Nkx3.1 antibody [EPR16653] (ab196020)

All lanes : Anti-Nkx3.1 antibody [EPR16653] (ab196020) at 1/1000 dilution

Lane 1 : LNCaP (Human prostate cancer cell line) cell lysate
Lane 2 : PC-3 (Human prostate cancer cell line) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 26 kDa
Observed band size: 32 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Lane 2, PC-3 (Human prostate cancer cell line) is acting as a negative control for Nkx3.1. The 32kDa band may represent the glycosylated form.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR16653] (ab196020)

Immunohistochemical analysis of paraffin-embedded Human prostate hyperplasia tissue labeling Nkx3.1 with ab196020 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human prostate hyperplasia tissue is observed. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Nkx3.1 antibody [EPR16653] (ab196020)

Anti-Nkx3.1 antibody [EPR16653] (ab196020) at 1/1000 dilution + Human prostate lysate at 10 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 26 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR16653] (ab196020)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Nkx3.1 with ab196020 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Human testis tissue acting as a negative control for Nkx3.1. Counter stained with Hematoxylin.

Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-Nkx3.1 antibody [EPR16653] (ab196020)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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