Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NKG2A antibody [EPR23737-127] - BSA and Azide free (ab273516)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23737-127] to NKG2A - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, ICC/IF, IP, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-NKG2A antibody [EPR23737-127] - BSA and Azide free
    See all NKG2A primary antibodies

  • Description

    Rabbit monoclonal [EPR23737-127] to NKG2A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, ICC/IF, IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NK-92 whole cell lysate; HEK-293 transfected with NKG2A (WT) expression vector containing a myc-His-tag, whole cell lysate. IHC-P: Human tonsil, NK cell lymphoma and gastric cancer tissue. ICC/IF: NK-92 cells. Flow Cyt: Human peripheral blood mononuclear cells and NK-92 cells. IP: NK-92 whole cell lysate.

  • General notes

    ab273516 is the carrier-free version of ab260035. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23737-127

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-NKG2A antibody [EPR23737-127] - BSA and Azide free (ab273516)
    Flow Cytometry - Anti-NKG2A antibody [EPR23737-127] - BSA and Azide free (ab273516)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized Jurkat (Human T cell leukemia T lymphocyte, Left) / NK-92 (Human malignant non-Hodgkin's lymphoma natural killer cell, Right) cells labelling NKG2A with ab260035 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Negative control: Jurkat (PMID: 15699146).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260035).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A antibody [EPR23737-127] - BSA and Azide free (ab273516)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A antibody [EPR23737-127] - BSA and Azide free (ab273516)

    Immunohistochemical analysis of paraffin-embedded Human NK cell lymphoma tissue labeling NKG2A with ab260035 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human NK cell lymphoma (PMID: 11222501).The section was incubated with ab260035 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260035).

  • Immunoprecipitation - Anti-NKG2A antibody [EPR23737-127] - BSA and Azide free (ab273516)
    Immunoprecipitation - Anti-NKG2A antibody [EPR23737-127] - BSA and Azide free (ab273516)

    NKG2A was immunoprecipitated from 0.35 mg NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) whole cell lysate with ab260035 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 260035 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell) whole cell lysate 10 ug

    Lane 2: 260035 IP in NK-92 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab260035 in NK-92 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 58 seconds.

    Multiple bands are likely due to isoforms and glycosylation. The molecular profile/weight observed is consistent with what has been described in the literature (PMID: 9034158).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260035).

  • Immunocytochemistry/ Immunofluorescence - Anti-NKG2A antibody [EPR23737-127] - BSA and Azide free (ab273516)
    Immunocytochemistry/ Immunofluorescence - Anti-NKG2A antibody [EPR23737-127] - BSA and Azide free (ab273516)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NK-92 cells labelling NKG2A with ab260035 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous and cytoplasmic staining in NK-92 cell line. Negative control: Jurkat (PMID: 15699146). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260035).

  • Flow Cytometry - Anti-NKG2A antibody [EPR23737-127] - BSA and Azide free (ab273516)
    Flow Cytometry - Anti-NKG2A antibody [EPR23737-127] - BSA and Azide free (ab273516)

    Flow cytometric analysis of 2% paraformaldehyde fixed, 0.1% Tween-20 permeabilized Human peripheral blood mononuclear cell (PBMC) cells labelling NKG2A with ab260035 at 1/500 dilution (0.1ug)/ Right  compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). 

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with anti-CD56 conjugated to PE, then fixed with 2% PFA followed by intracellularly stained with rabbit IgG (Left) or ab260035 (Right).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260035).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A antibody [EPR23737-127] - BSA and Azide free (ab273516)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A antibody [EPR23737-127] - BSA and Azide free (ab273516)

    Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling NKG2A with ab260035 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human gastric cancer (PMID: 11222501).The section was incubated with ab260035 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260035).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A antibody [EPR23737-127] - BSA and Azide free (ab273516)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2A antibody [EPR23737-127] - BSA and Azide free (ab273516)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling NKG2A with ab260035 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil (PMID: 11222501). The section was incubated with ab260035 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab260035).

  • Anti-NKG2A antibody [EPR23737-127] - BSA and Azide free (ab273516)
    Anti-NKG2A antibody [EPR23737-127] - BSA and Azide free (ab273516)

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