Antibodies, Proteins, Kits and Reagents for Life Science

288 134 ₸ Product size

100 µl 288 134 ₸

Order now and get it on Wednesday March 03, 2021

Anti-CD16 antibody [EPR20537] (ab211151)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20537] to CD16
Suitable for: WB, ICC/IF
Reacts with: Rat, Human

Product name

Anti-CD16 antibody [EPR20537]
See all CD16 primary antibodies
Description

Rabbit monoclonal [EPR20537] to CD16
Host species

Rabbit
Specificity

We recommend ICC for human only.
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal brain lysate; Rat brain and P0 retina lysates; C6, U-937, THP-1, Jurkat, K-562 and PC-12 whole cell lysates. ICC/IF: Jurkat cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
ICC/IF	Human
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20537
Isotype

IgG
Research areas

Western blot - Anti-CD16 antibody [EPR20537] (ab211151)

All lanes : Anti-CD16 antibody [EPR20537] (ab211151) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Rat brain lysate
Lane 3 : C6 (rat glial tumor cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 29 kDa
Observed band size: 50 kDa
why is the actual band size different from the predicted?

Exposure time : Lane 1: 26 seconds; Lane 2: 70 seconds; Lane 3: 3 minutes.

Blocking and dilution buffer: 5% NFDM/TBST.

The molecular mass observed is consistent with what has been described in the literature (PMID: 28652325).
Western blot - Anti-CD16 antibody [EPR20537] (ab211151)

All lanes : Anti-CD16 antibody [EPR20537] (ab211151) at 1/1000 dilution

Lane 1 : U937 (human histiocytic lymphoma cell line) whole cell lysate
Lane 2 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 5 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 6 : Rat P0 retina lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 29 kDa

Exposure time : Lanes 1 and 2: 3 minutes; Lanes 3-6: 37 seconds.

Blocking and dilution buffer: 5% NFDM/TBST.

The molecular mass observed is consistent with what has been described in the literature (PMID: 28652325).
Immunocytochemistry/ Immunofluorescence - Anti-CD16 antibody [EPR20537] (ab211151)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling CD16 with ab211151 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in Jurkat cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Anti-CD16 antibody [EPR20537] (ab211151)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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