Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AHSG antibody [EPR17839-163] - BSA and Azide free (ab226243)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17839-163] to AHSG - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-AHSG antibody [EPR17839-163] - BSA and Azide free
    See all AHSG primary antibodies

  • Description

    Rabbit monoclonal [EPR17839-163] to AHSG - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse liver tissue.

  • General notes

    Ab226243 is the carrier-free version of ab187051. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab226243 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-AHSG antibody [EPR17839-163] - BSA and Azide free (ab226243)
    Immunoprecipitation - Anti-AHSG antibody [EPR17839-163] - BSA and Azide free (ab226243)

    AHSG was immunoprecipitated from 0.35 mg of mouse serum with ab187051 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187051 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: Mouse serum 10 μg (Input).
    Lane 2: ab187051 IP in mouse serum (+).
    Lane 3: : Rabbit monoclonal IgG (ab172730) instead of ab187051 in mouse serum (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187051).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHSG antibody [EPR17839-163] - BSA and Azide free (ab226243)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHSG antibody [EPR17839-163] - BSA and Azide free (ab226243)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling AHSG with ab187051 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat liver (PMID: 24604240; PMID: 12943536; PMID: 22170794) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187051).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHSG antibody [EPR17839-163] - BSA and Azide free (ab226243)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHSG antibody [EPR17839-163] - BSA and Azide free (ab226243)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling AHSG with ab187051 at 1/2,000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse liver (PMID: 24604240; PMID: 12943536; PMID: 22170794) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187051).

  • Anti-AHSG antibody [EPR17839-163] - BSA and Azide free (ab226243)
    Anti-AHSG antibody [EPR17839-163] - BSA and Azide free (ab226243)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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