Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-AHSG antibody [EPR17839-163] (ab187051)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17839-163] to AHSG
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-AHSG antibody [EPR17839-163]
    See all AHSG primary antibodies

  • Description

    Rabbit monoclonal [EPR17839-163] to AHSG

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    IP
    Mouse
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat serum and plasma; rat liver tissue lysate. IHC-P: Mouse and rat liver tissue. IP: Mouse serum.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-AHSG antibody [EPR17839-163] (ab187051)
    Western blot - Anti-AHSG antibody [EPR17839-163] (ab187051)
    All lanes : Anti-AHSG antibody [EPR17839-163] (ab187051) at 1/2000 dilution

    Lane 1 : Mouse serum
    Lane 2 : Mouse plasma
    Lane 3 : Rat serum
    Lane 4 : Rat plasma

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

    Developed using the ECL technique.

    Predicted band size: 37 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking and dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHSG antibody [EPR17839-163] (ab187051)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHSG antibody [EPR17839-163] (ab187051)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling AHSG with ab187051 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on mouse liver (PMID: 24604240; PMID: 12943536; PMID: 22170794) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunoprecipitation - Anti-AHSG antibody [EPR17839-163] (ab187051)
    Immunoprecipitation - Anti-AHSG antibody [EPR17839-163] (ab187051)

    AHSG was immunoprecipitated from 0.35 mg of mouse serum with ab187051 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab187051 at 1/1,000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: Mouse serum 10 μg (Input).
    Lane 2: ab187051 IP in mouse serum (+).
    Lane 3: : Rabbit monoclonal IgG (ab172730) instead of ab187051 in mouse serum (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 1 second.

  • Western blot - Anti-AHSG antibody [EPR17839-163] (ab187051)
    Western blot - Anti-AHSG antibody [EPR17839-163] (ab187051)
    Anti-AHSG antibody [EPR17839-163] (ab187051) at 1/2000 dilution + Rat liver tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

    Developed using the ECL technique.

    Predicted band size: 37 kDa
    Observed band size: 58 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking and dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHSG antibody [EPR17839-163] (ab187051)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AHSG antibody [EPR17839-163] (ab187051)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling AHSG with ab187051 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on rat liver (PMID: 24604240; PMID: 12943536; PMID: 22170794) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-AHSG antibody [EPR17839-163] (ab187051)
    Anti-AHSG antibody [EPR17839-163] (ab187051)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-AHSG antibody [EPR17839-163] (ab187051)

© 2021 Astana Biomed Group LLP. All rights reserved.