Anti-NKG2A antibody [EPR23620-39] (ab252437)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23620-39] to NKG2A
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-NKG2A antibody [EPR23620-39]
See all NKG2A primary antibodies -
Description
Rabbit monoclonal [EPR23620-39] to NKG2A -
Host species
Rabbit -
Specificity
This product is not recommend for use in ICC and IHC. Internal testing using over expressing cell lines has shown this product cross-reacts with NKG2C in these applications. Cross-reactivity with NKG2E was not observed.
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Tested Applications & Species
See all applications and species dataApplication Species WB HumanRecombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with NKG2-A (WT) expression vector containing a myc-His-tag®, whole cell lysate.
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General notes
We have some reports that this antibody does not provide good results in IHC-P. Please contact our Scientific Support team if you have data using ab252437 in this application.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23620-39 -
Isotype
IgG -
Research areas
Images
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Anti-NKG2A antibody [EPR23620-39] (ab252437) at 1/1000 dilution + NK-92 (human malignant non-Hodgkin's lymphoma natural killer cell), whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 26 kDa
Observed band size: 34-38 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Expossure time: 3 minutes.
This blot was developed using a higher sensitivity ECL substrate.
Multiple bands are likely due to isoforms and glycosylation. The molecular profile/weight observed is consistent with what has been described in the literature (PMID: 9034158).
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All lanes : Anti-NKG2A antibody [EPR23620-39] (ab252437) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with NKG2-A (WT) expression vector containing a myc-His-tag®, whole cell lysate
Lanes 3 & 5 : HEK-293T transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 4 : HEK-293T transfected with NKG2-C (WT) expression vector containing a myc-His-tag®, whole cell lysate
Lane 6 : HEK-293T transfected with NKG2-E (WT) expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 26 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
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