Anti-NG2 antibody [7.1] (ab279348)
![Anti-NG2 antibody [7.1] (ab279348)](https://www.abcam.com/ps/products/279/ab279348/Images/ab279348-2-anti-ng2-antibody-71-flow-cytometry-skbr3.jpg "Anti-NG2 antibody [7.1] (ab279348)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [7.1] to NG2
- Suitable for: ICC, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-NG2 antibody [7.1]
See all NG2 primary antibodies -
Description
Mouse monoclonal [7.1] to NG2 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: SK-MEL-28 cells. Flow Cyt: SK-MEL-28 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
7.1 -
Isotype
IgG1 -
Research areas
Images
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Flow Cytometry - Anti-NG2 antibody [7.1] (ab279348)
Flow cytometric analysis of SK-BR-3 (Human breast adenocarcinoma epithelial cell, Left) / SK-MEL-28 (Human malignant melanoma, Right) cells labelling NG2 with ab279348 at 1/1000 dilution (0.1µg) (Red) compared with a Mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
Negative control: SK-BR-3.
Gated on viable cells.
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![Immunocytochemistry - Anti-NG2 antibody [7.1] (ab279348)](https://www.abcam.com/ps/products/279/ab279348/Images/ab279348-1-anti-ng2-antibody-71-immunocytochemistry-skmel28.jpg)
Immunocytochemistry - Anti-NG2 antibody [7.1] (ab279348)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-MEL-28 cells labelling NG2 with ab279348 at 1/100 (11.02 µg/ml) dilution, followed by ab150113 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in SK-MEL-28 cells.
Negative control: SK-BR-3 (PMID: 20852124).
ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab271294 at a 1/100 dilution followed by ab150080 at a 1/1000 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150157 at a 1/1000 dilution.
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![Anti-NG2 antibody [7.1] (ab279348)](https://www.abcam.com/ps/products/279/ab279348/Images/ab279348-456297-benefitsofrecombinantantibodies.jpg)
Anti-NG2 antibody [7.1] (ab279348)
